Poly-ether-ether-ketone (PEEK) implants with good mechanical properties and chemical inertia, meet the urgent needs of bone substitute. However, its inert interface leads to poor soft tissue integration, which prolongs healing time of surgical incision with many complications. Herein, (3-aminopropyl) triethoxysilane is connected to 3D printed (3DP) PEEK interface by chemical modification. The homogeneous amino groups on amidogen interface enhance PEEK's hydrophilicity and proteinophilia significantly. Fibroblasts cultured on the amidogen PEEK interface show much stronger potential of cell adhesion and migration. Furthermore, soft tissue ingrowth into 3DP PEEK scaffold occurs more and faster in the amidogen interface in vivo. The observation of the microstructure shows tighter implant–tissue bonding interfaces on the amidogen PEEK. To mimic real surgery, 3DP PEEK implants of the same proportions in clinical practice are used to reconstruct the chest wall defects of rabbits. A significant reduction in healing time and incision complications are observed in the amidogen PEEK groups. In addition, 19 related proteins are found in the fibroblasts cultured on the amidogen PEEK interface, which can be used to trace the biological mechanisms. In all, the facile amidogen bio-activation method can significantly boost the soft tissue integration on 3DP PEEK interface with less surgical complications.

中文翻译：

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简便的 Amidogen 生物活化方法可以促进 3D 打印聚醚醚酮界面上的软组织整合

聚醚醚酮（PEEK）植入物具有良好的机械性能和化学惰性，满足了骨替代品的迫切需求。然而，其惰性界面导致软组织整合不良，延长了手术切口的愈合时间，并伴有许多并发症。在此，（3-氨基丙基）三乙氧基硅烷通过化学修饰连接到 3D 打印（3DP）PEEK 界面。胺基界面上的均质氨基显着增强了 PEEK 的亲水性和亲蛋白性。在amidogen PEEK 界面上培养的成纤维细胞显示出更强的细胞粘附和迁移潜力。此外，软组织向 3DP PEEK 支架的向内生长在体内酰胺基界面中发生得越来越快。微观结构的观察表明，amidogen PEEK 上的植入物-组织结合界面更紧密。为了模拟真实手术，在临床实践中使用相同比例的3DP PEEK植入物重建兔子的胸壁缺损。在amidogen PEEK 组中观察到愈合时间和切口并发症的显着减少。此外，在amidogen PEEK界面上培养的成纤维细胞中发现了19种相关蛋白，可用于追踪生物学机制。总之，简便的酰胺基生物活化方法可以显着促进 3DP PEEK 界面上的软组织整合，同时减少手术并发症。在amidogen PEEK界面上培养的成纤维细胞中发现了19种相关蛋白，可用于追踪生物学机制。总之，简便的酰胺基生物活化方法可以显着促进 3DP PEEK 界面上的软组织整合，同时减少手术并发症。在amidogen PEEK界面上培养的成纤维细胞中发现了19种相关蛋白，可用于追踪生物学机制。总之，简便的酰胺基生物活化方法可以显着促进 3DP PEEK 界面上的软组织整合，同时减少手术并发症。