There is an urgent clinical need for wound dressings to treat skin injuries, particularly full-thickness wounds caused by acute and chronic wounds. Marine collagen has emerged as an attractive and safer alternative due to its biocompatibility, diversity, and sustainability. It has minimum risk of zoonotic diseases and less religious constraints as compared to mammalian collagen. In this study, we reported the development of a self-assembled nanofibrous barramundi (Lates calcarifer) collagen matrix (Nano-BCM), which showed good biocompatibility for full-thickness wound-healing applications. The collagen was extracted and purified from barramundi scales and skin. Thereafter, the physicochemical properties of collagen were systematically evaluated. The process to extract barramundi skin collagen (BC) gave an excellent 45% yield and superior purity (∼100%). More importantly, BC demonstrated structural integrity, native triple helix structure, and good thermal stability. BC demonstrated its efficacy in promoting human primary dermal fibroblast (HDF) and immortalized human keratinocytes (HaCaT) proliferation and migration. Nano-BCM has been prepared via self-assembly of collagen molecules in physiological conditions, which resembled the native extracellular matrix (ECM). The clinical therapeutic efficacy of the Nano-BCM was further evaluated in a full-thickness splinted skin wound mice model. In comparison to a clinically used wound dressing (DuoDerm), the Nano-BCM demonstrated significantly accelerated wound closure and re-epithelization. Moreover, Nano-BCM nanofibrous architecture and its ability to facilitate early inflammatory response significantly promoted angiogenesis and differentiated myofibroblast, leading to enhanced wound healing. Consequently, Nano-BCM demonstrates great potential as an economical and effective nonmammalian substitute to achieve skin regeneration.

中文翻译：

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自组装纳米纤维海洋胶原蛋白基质加速全层伤口愈合

临床迫切需要伤口敷料来治疗皮肤损伤，特别是由急性和慢性伤口引起的全层伤口。由于其生物相容性、多样性和可持续性，海洋胶原蛋白已成为一种有吸引力且更安全的替代品。与哺乳动物胶原蛋白相比，它患人畜共患病的风险最小，宗教限制也更少。在这项研究中，我们报道了一种自组装纳米纤维尖吻鲈（Lates calcarifer) 胶原蛋白基质 (Nano-BCM)，在全层伤口愈合应用中表现出良好的生物相容性。从澳洲肺鱼鳞片和皮肤中提取和纯化胶原蛋白。此后，系统地评估了胶原蛋白的理化性质。提取澳洲肺鱼皮肤胶原蛋白 (BC) 的过程产生了出色的 45% 产率和卓越的纯度 (~100%)。更重要的是，BC 表现出结构完整性、天然三螺旋结构和良好的热稳定性。BC 证明了其在促进人原代真皮成纤维细胞 (HDF) 和永生化人角质形成细胞 (HaCaT) 增殖和迁移方面的功效。Nano-BCM 是通过胶原分子在生理条件下的自组装制备的，类似于天然细胞外基质 (ECM)。Nano-BCM 的临床疗效在全层夹板皮肤伤口小鼠模型中得到进一步评估。与临床使用的伤口敷料 (DuoDerm) 相比，Nano-BCM 表现出显着加速伤口闭合和上皮再生。此外，Nano-BCM 纳米纤维结构及其促进早期炎症反应的能力显着促进血管生成和分化的肌成纤维细胞，从而促进伤口愈合。因此，Nano-BCM 作为一种经济有效的非哺乳动物替代品，在实现皮肤再生方面表现出巨大的潜力。Nano-BCM 纳米纤维结构及其促进早期炎症反应的能力显着促进血管生成和分化的肌成纤维细胞，从而促进伤口愈合。因此，Nano-BCM 作为一种经济有效的非哺乳动物替代品，在实现皮肤再生方面表现出巨大的潜力。Nano-BCM 纳米纤维结构及其促进早期炎症反应的能力显着促进血管生成和分化的肌成纤维细胞，从而促进伤口愈合。因此，Nano-BCM 作为一种经济有效的非哺乳动物替代品，在实现皮肤再生方面表现出巨大的潜力。