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Sensitive Mercury Speciation Analysis in Water by High-Performance Liquid Chromatography–Atomic Fluorescence Spectrometry Coupling with Solid-Phase Extraction
Analytical Sciences ( IF 1.6 ) Pub Date : 2021-09-10 , DOI: 10.2116/analsci.20p398
Dongyang Chen 1 , Lan Lu 1 , Hao Zhang 1 , Bing Lu 1 , Jiali Feng 1 , Dong Zeng 1
Affiliation  

An efficient method based on high-performance liquid chromatography coupled with atomic fluorescence spectrometry (HPLC-AFS) was successfully developed for the simultaneous determination of four mercury species including Hg2+, methylmercury (MeHg), ethylmercury (EtHg), and phenylmercury (PhHg) in water. Samples were enriched and cleaned up with a solid-phase extraction (SPE) pretreatment using a thiol cartridge. Some key parameters including the selection of a SPE cartridge, eluent type, eluent volume, and interference factors were systematically investigated. Chromatographic separation was achieved on a C18 column using a mobile phase consisting of methanol and 60 mmol L−1 ammonium acetate with 10 mmol L−1 L-cysteine by gradient elution. Under the optimized conditions, good linearity (r ≥ 0.9991) was observed between 0.20 to 10.0 μg L−1. The limits of detection were in the range of 0.001 – 0.002 μg L−1. High recoveries (87.2 to 111%) and good reproducibility (1.1 – 6.5%) were obtained. Such a method is sensitive, selective and accurate, which can be applied to the quantification of mercury species in water samples.

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中文翻译:

使用高效液相色谱-原子荧光光谱法结合固相萃取法对水中的敏感汞形态进行分析

成功开发了一种基于高效液相色谱-原子荧光光谱法 (HPLC-AFS) 的高效方法,用于同时测定 Hg 2+、甲基汞 (MeHg)、乙基汞 (EtHg) 和苯基汞 (PhHg)四种汞) 在水里。使用硫醇小柱通过固相萃取 (SPE) 预处理对样品进行富集和净化。系统研究了一些关键参数,包括 SPE 小柱的选择、洗脱液类型、洗脱液体积和干扰因素。在 C 18柱上使用由甲醇和 60 mmol L -1乙酸铵组成的流动相实现色谱分离,其中 10 mmol L -1L-半胱氨酸通过梯度洗脱。在优化条件下,在 0.20 至 10.0 μg L -1之间观察到良好的线性 ( r ≥ 0.9991) 。检测限在 0.001 – 0.002 μg L -1的范围内。获得了高回收率 (87.2 至 111%) 和良好的重现性 (1.1 – 6.5%)。该方法灵敏度高、选择性好、准确度高,可用于水样中汞种类的定量分析。

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更新日期:2021-09-10
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