Paper spray mass spectrometry is an ambient ionization technique capable of the direct ionization of analyte from a bio-fluid spot on a paper substrate. Different solvents and types of paper can have different interactions with the analytes of interest; therefore, they can significantly impact the analyte signal and the assay as a whole. In this study, we examined the effects of substrate-solvent composition on signal intensity, blank signal intensity, and signal-to-blank ratio for a variety of pharmaceutical drugs, illicit drugs, chemical warfare agent (CWA) simulants, and CWA hydrolysis products. The analytes were prepared either neat or spiked into human plasma and deposited on a variety of modified paper substrates. Extraction occurred with a range of different solvents. Optimizing the substrate-solvent combination improved the signal-to-blank ratio for all compounds ranging from 1 to 7,964 factor improvement, with the substrate providing a more impactful improvement. Aprotic solvents, such as tetrahydrofuran and ethyl acetate, tended to produce optimal signal-to-blank ratios, while carbon sputtered and glass fiber substrates were the top paper substrates. The research presented herein illustrates the need for systematic optimization of substrate and spray solvent combinations to achieve the best detection limits for paper spray analysis.

纸喷雾质谱是一种环境电离技术，能够从纸基材上的生物流体点直接电离分析物。不同的溶剂和纸张类型可能与目标分析物有不同的相互作用；因此，它们可以显着影响分析物信号和整个检测。在本研究中，我们检查了底物 - 溶剂组成对各种药物、违禁药物、化学战剂 (CWA) 模拟物和 CWA 水解产物的信号强度、空白信号强度和信空白比的影响. 分析物制备成纯净物或掺入人血浆中并沉积在各种改性纸基材上。用一系列不同的溶剂进行萃取。优化底物-溶剂组合改善了所有化合物的信空白比，提高了 1 到 7,964 个因子，底物提供了更有效的改进。非质子溶剂，如四氢呋喃和乙酸乙酯，往往会产生最佳的信空白比，而碳溅射和玻璃纤维基材是顶级纸基材。此处介绍的研究表明，需要对基材和喷雾溶剂组合进行系统优化，以实现纸喷雾分析的最佳检测限。而碳溅射和玻璃纤维基材是顶层纸基材。此处介绍的研究表明，需要对基材和喷雾溶剂组合进行系统优化，以实现纸喷雾分析的最佳检测限。而碳溅射和玻璃纤维基材是顶层纸基材。此处介绍的研究表明，需要对基材和喷雾溶剂组合进行系统优化，以实现纸喷雾分析的最佳检测限。