Toxicological effects of herbicides on the non-target microorganisms in the soil that play important roles in degrading organic matter, nutrient cycling, and decomposition should be considered if they were applied at higher concentrations. This study was conducted to reveal the toxicity of three modern herbicides (dicamba + tritosulfuron; DT, isoxinutole + thlencarbuone-methyl + cyprosulfamide; ITC and nicosulfuron; N) on soil carbon mineralization for short term. Recommended concentration (25 g/day for DT1, 30 ml/day for ITC1, and 125 ml/day N1), its 5 (DT5, ITC5, and N5), and 10 (DT10, ITC10, and N10) folds of herbicides were applied on a clay soil with no pesticide application history. Soil + herbicide mixes and soil without herbicide as control were incubated under constant temperature (28 °C) and moisture (80% of soil field capacity) for 42 days. In general, all doses of herbicides were significantly decreased soil carbon mineralization in short term (P < 0.05). Cumulative carbon mineralization was declined for 3.4% by DT1, for 15.0% by DT5, for 22.4% by DT10, for 13.7% by ITC1, for 14.2% by ITC5, for 18.6% by ITC10, for 4.7% by N1, for 10.0% by N5, and for 21.0% by N10, respectively, compared to control. Significant decreases were observed in the rates of carbon mineralization except DT1 and N1. Results suggested that higher doses of these herbicides negatively affected on soil microbial respiration.

如果除草剂以较高浓度施用，则应考虑除草剂对土壤中在降解有机物、养分循环和分解中起重要作用的非目标微生物的毒理学影响。本研究旨在揭示三种现代除草剂（麦草畏+三磺隆；DT，异茚酮+甲基噻嘧磺隆；ITC和烟嘧磺隆；N）对土壤碳矿化的短期毒性。推荐浓度（DT1 为 25 克/天，ITC1 为 30 毫升/天，N1 为 125 毫升/天），其 5（DT5、ITC5 和 N5）和 10（DT10、ITC10 和 N10）倍除草剂为施用在没有农药施用历史的粘土上。将土壤 + 除草剂混合物和不含除草剂的土壤作为对照，在恒温 (28 °C) 和湿度（土壤田间容量的 80%）下培养 42 天。一般而言，短期内所有剂量的除草剂均显着降低土壤碳矿化（P  < 0.05)。DT1累计碳矿化下降3.4%，DT5下降15.0%，DT10下降22.4%，ITC1下降13.7%，ITC5下降14.2%，ITC10下降18.6%，N1下降4.7%，10%。与对照相比，分别为 N5 和 N10 的 21.0%。除 DT1 和 N1 外，碳矿化速率显着下降。结果表明，较高剂量的这些除草剂会对土壤微生物呼吸产生负面影响。