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3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton’s Jelly Mesenchymal Stem Cells
Pharmaceutics ( IF 5.4 ) Pub Date : 2021-09-10 , DOI: 10.3390/pharmaceutics13091448
Maria Camilla Ciardulli 1 , Joseph Lovecchio 2 , Pasqualina Scala 1 , Erwin Pavel Lamparelli 1 , Tina Patricia Dale 3 , Valentina Giudice 1, 4 , Emanuele Giordano 2, 5, 6 , Carmine Selleri 1, 4, 7 , Nicholas Robert Forsyth 3 , Nicola Maffulli 1, 3, 8 , Giovanna Della Porta 1, 9
Affiliation  

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

中文翻译:

用于生长因子控制递送的 3D 仿生支架:沃顿氏果冻间充质干细胞肌腱发生事件的体外研究

目前的工作描述了一种生物功能化的 3D 纤维结构,作为一种交互式肌腱诱导移植模型,用于研究从沃顿氏果冻 (hWJ-MSCs) 收集的人类间充质干细胞的肌腱潜在事件。3D 仿生和生物可吸收支架用纳米载体功能化,用于局部控制递送腱诱导因子,即人类生长分化因子 5 (hGDF-5)。获得了基因表达方面的显着结果。即,在第 14 天检测到 Scleraxis(350 倍,p ≤ 0.05)、I 型胶原蛋白(8 倍)、核心蛋白聚糖(2.5 倍)和 Tenascin-C(1.3 倍)的上调;另一方面,当 hGDF-5 仅在外部培养基中补充时(没有纳米载体),对基因表达的影响很明显。Teno-inductive 环境也诱导了促炎,
更新日期:2021-09-10
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