In the present study, the polysaccharide-hydrolyzing secretomes of Irpex lacteus (Fr.) Fr. (1828) BCC104, Pycnoporus coccineus (Fr.) Bondartsev and Singer (1941) BCC310, and Schizophyllum commune Fr. (1815) BCC632 were analyzed in submerged fermentation conditions to elucidate the effect of chemically and structurally different carbon sources on the expression of cellulases and xylanase. Among polymeric substrates, crystalline cellulose appeared to be the best carbon source providing the highest endoglucanase, total cellulase, and xylanase activities. Mandarin pomace as a growth substrate for S. commune allowed to achieve comparatively high volumetric activities of all target enzymes while wheat straw induced a significant secretion of cellulase and xylanase activities of I. lacteus and P. coccineus. An additive effect on the secretion of cellulases and xylanases by the tested fungi was observed when crystalline cellulose was combined with mandarin pomace. In I. lacteus the cellulase and xylanase production is inducible in the presence of cellulose-rich substrates but is suppressed in the presence of an excess of easily metabolizable carbon source. These enzymes are expressed in a coordinated manner under all conditions studied. It was shown that the substitution of glucose in the inoculum medium with Avicel provides accelerated enzyme production by I. lacteus and higher cellulase and xylanase activities of the fungus. These results add new knowledge to the physiology of basidiomycetes to improve cellulase production.

中文翻译：

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碳源控制担子菌多糖水解酶的分泌和产量

在本研究中，Irpex lacteus (Fr.) Fr.的多糖水解分泌组。(1828) BCC104、Pycnoporus coccineus (Fr.) Bondartsev 和 Singer (1941) BCC310 和Schizophyllum commune Fr. (1815) BCC632 在深层发酵条件下进行了分析，以阐明化学和结构不同的碳源对纤维素酶和木聚糖酶表达的影响。在聚合物基质中，结晶纤维素似乎是提供最高内切葡聚糖酶、总纤维素酶和木聚糖酶活性的最佳碳源。普通话渣作为S. commune的生长基质允许实现所有目标酶的相对较高的体积活性，而小麦秸秆诱导I. lacteus和P. coccineus的纤维素酶和木聚糖酶活性的显着分泌。当结晶纤维素与柑橘渣混合时，观察到对测试真菌分泌纤维素酶和木聚糖酶的累加效应。在I. lacteus中，纤维素酶和木聚糖酶的产生在富含纤维素的底物存在下是可诱导的，但在容易代谢的碳源过量存在时会受到抑制。这些酶在所研究的所有条件下都以协调的方式表达。结果表明，用 Avicel 代替接种培养基中的葡萄糖可通过以下方式加速酶的产生I. lacteus和高等纤维素酶和木聚糖酶活性的真菌。这些结果为担子菌的生理学增加了新的知识，以改善纤维素酶的产生。