Our previous study showed that the water-soluble heteropolysaccharide extracted from Gracilaria lemaneiformis (GLHP) has excellent anti-inflammation and anti-oxidant properties. This study explored the efficacy of GLHP against skin anti-photoaging in human immortalized keratinocytes (HaCaT) cells and BALB/c mice under UVB irradiation. Cell viability, antiapoptotic, reactive oxygen species (ROS) scavenging activity, mitochondrial membrane potential, and cell wound scratch assays were conducted, as well as assessment of inflammation markers and sun protection factors. The in vitro results showed that GLHP pretreatment significantly inhibited UVB-induced apoptosis, reversed the decrease of cell viability via downregulating the expression of apoptosis-related protein caspase-3, accelerated the migration of HaCaT cells, and promoted wound healing. Notably, the protective effect of GLHP may be associated with the scavenging of ROS and the decrease of mitochondrial membrane potential. Moreover, GLHP pretreatment significantly restrained the upregulation of iNOS (UVB-induced inflammation marker), suppressed the expression of P-ERK and NF-κB, and decreased the activity of MMPs, suggesting that it exerts the therapeutic effects by inhibiting the MAPK/NF-κB signal pathway. Results obtained after conducting the in vivo assay confirmed that GLHP could reverse the UVB-induced increase of epidermal thickness in BALB/c mice. In conclusion, this study shows that GLHP can be utilized as a safer resource in the manufacture of anti-aging cosmetics because it exerts excellent anti-photoaging effects.

我们之前的研究表明，从江蓠（GLHP）中提取的水溶性杂多糖具有优异的抗炎和抗氧化特性。本研究探讨了 GLHP 在 UVB 照射下对人永生化角质形成细胞 (HaCaT) 细胞和 BALB/c 小鼠的皮肤抗光老化的功效。进行了细胞活力、抗细胞凋亡、活性氧 (ROS) 清除活性、线粒体膜电位和细胞伤口划痕试验，以及炎症标志物和防晒因子的评估。的体外结果表明，GLHP预处理显著抑制UVB诱导的细胞凋亡，逆转细胞活力的减少经由下调凋亡相关蛋白 caspase-3 的表达，加速 HaCaT 细胞的迁移，促进伤口愈合。值得注意的是，GLHP 的保护作用可能与清除 ROS 和降低线粒体膜电位有关。此外，GLHP预处理显着抑制了iNOS（UVB诱导的炎症标志物）的上调，抑制了P-ERK和NF-κB的表达，降低了MMPs的活性，表明其通过抑制MAPK/NF发挥治疗作用。 -κB 信号通路。进行体内试验后获得的结果测定证实 GLHP 可以逆转 UVB 诱导的 BALB/c 小鼠表皮厚度增加。总之，这项研究表明，GLHP 可以作为一种更安全的资源用于制造抗衰老化妆品，因为它具有出色的抗光老化作用。