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Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests
Journal of King Saud University-Science ( IF 3.8 ) Pub Date : 2021-09-09 , DOI: 10.1016/j.jksus.2021.101595
Majesh Tomson 1, 2 , Kitherian Sahayaraj 1 , Samy Sayed 3 , Sayed-Ashraf Elarnaouty 4 , Ganeshan Petchidurai 1
Affiliation  

Entomopathogenic fungi are widely used as biocontrol agents against several agricultural pests. Among them, Beauveria bassiana is considered the important one against insect and other arthropod pests. The entomotoxic proteins of B. bassiana were extracted by Sephadex G-25 column, and fractionated using HPLC (BBI, BBII and BBIII) and tested against two hemipteran insect pests i.e., Dysdercus cingulatus Fab. and Phenacoccus solenopsis Tinsely (Hemiptera: Pseudococcidae). Results indicated that protein content was higher in fraction BBII than BBI and BBIII. The vibration frequency in FT-IR obtained with a range of 1650 to 1580 cm−1. Bioassays of fractions (I, II and III) reveal that BBII was highly virulent against third nymphal instar of D. cingulatus (LC50 = 800.2 ppm) and adults of P. solenopsis adult (LC50 = 713.3 ppm). Considering the high virulence of BBII subjected to SDS-PAGE, HPLC and MALDI-TOF analyses. Analyses reveals the presence of 174 kDa and designated as BBF2. These results concluded that the entomotoxic protein of B. bassiana can be utilized for management of these investigated hemipreran pests. Further investigations are necessary for the field application of this entomotoxin against these pests or other insect pests. These results also could be helpful for establishing novel biotechnological uses for this fungus.



中文翻译:

白僵菌的昆虫毒性蛋白。(Vuil.) 及其对两种棉花害虫的毒力

昆虫病原真菌被广泛用作对抗几种农业害虫的生物防治剂。其中白僵菌被认为是防治昆虫和其他节肢动物害虫的重要品种。的entomotoxic蛋白白僵菌物经Sephadex G-25柱中提取,并用HPLC(BBI,BBII和BBIII)分馏和针对两种半翅目昆虫害虫测试。,Dysdercus cingulatus的Fab。和Phenacoccus solenopsis Tinsely (半翅目:Pseudococcidae) 结果表明,BBII 级分中的蛋白质含量高于 BBI 和 BBIII。在 1650 至 1580 cm -1范围内获得的 FT-IR 振动频率. 级分 (I、II 和 III) 的生物测定表明 BBII 对D. cingulatus 的第三龄幼虫(LC 50  = 800.2 ppm) 和P. solenopsis成虫 (LC 50  = 713.3 ppm) 的成虫具有高度毒性。考虑到 BBII 经受 SDS-PAGE、HPLC 和 MALDI-TOF 分析的高毒力。分析显示存在 174 kDa 并指定为 BBF2。这些结果的结论是,entomotoxic蛋白白僵菌可以用于这些研究hemipreran害虫管理。对于这种昆虫毒素对这些害虫或其他害虫的田间应用,有必要进行进一步的调查。这些结果也可能有助于为这种真菌建立新的生物技术用途。

更新日期:2021-09-23
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