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The level of secondary messengers and the redox state of NAD+/NADH are associated with sperm quality in infertility
Journal of Reproductive Immunology ( IF 3.4 ) Pub Date : 2021-09-10 , DOI: 10.1016/j.jri.2021.103383
Shamil N Galimov 1 , Julia Y Gromenko 2 , Kirill V Bulygin 3 , Kamil Sh Galimov 4 , Elmira F Galimova 1 , Mikhail Y Sinelnikov 5
Affiliation  

In order to explore the interrelation of Calcium, cAMP, and redox state of pyridine nucleotides in seminal plasma and ejaculate quality in cases of idiopathic infertility we conducted an evaluation of 170 infertile males and 46 fertile males aged 20–43 years. Sperm analysis was undertaken according to WHO protocol. The content of Calcium in the seminal plasma was detected using optical emission spectrometry, cAMP levels were determined via enzymatic immunoassay. The redox state of pyridine nucleotides was evaluated from the ratio of pyruvate to lactate, determined via enzymatic method. Our results show a decrease in Calcium, cAMP, pyruvate and the oxidation-reduction potential of pyridine nucleotides in the seminal plasma of infertile males with pathospermia. This corresponds to anaerobic inversion of oxidative conversions and metabolism inadaptation. Such processes are often seen in inflammatory and autoimmune conditions. cAMP levels reliably correlated with the number of progressively mobile sperm cells, but not with the number of their pathological forms. A positive correlation between the concentration of cAMP and calcium was discovered as well. Pathospermia was characterized by the positive relation between the value of the NAD+/NADH coefficient and the spermatozoa concentration that was not present in fertile donors. Our study shows distinct changes in the concentration of secondary messengers and redox state of pyridine nucleotides in the seminal fluid that can act as molecular predictors for the development of idiopathic infertility.



中文翻译:

次级信使的水平和NAD+/NADH的氧化还原状态与不孕症的精子质量有关

为了探索精浆中钙、cAMP 和吡啶核苷酸的氧化还原状态的相互关系,以及在特发性不孕症病例中的射精质量,我们对 170 名不育男性和 46 名年龄在 20-43 岁的可育男性进行了评估。精子分析是根据 WHO 协议进行的。用发射光谱法检测精浆中钙的含量,用酶免疫法测定cAMP水平。吡啶核苷酸的氧化还原状态是​​通过酶法测定的丙酮酸与乳酸的比率来评估的。我们的结果显示,患有精子症的不育男性精浆中的钙、cAMP、丙酮酸和吡啶核苷酸的氧化还原电位降低。这对应于氧化转化和代谢不适应的厌氧反转。这种过程经常出现在炎症和自身免疫疾病中。cAMP 水平与逐渐移动的精子细胞数量可靠相关,但与其病理形式的数量无关。还发现了 cAMP 浓度与钙之间的正相关关系。精子症的特点是 NAD 值之间存在正相关关系+ /NADH 系数和可育供体中不存在的精子浓度。我们的研究表明,精液中次级信使的浓度和吡啶核苷酸的氧化还原状态发生了明显变化,这些变化可以作为特发性不孕症发展的分子预测因子。

更新日期:2021-09-14
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