当前位置: X-MOL 学术Acta Trop. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation
Acta Tropica ( IF 2.7 ) Pub Date : 2021-09-09 , DOI: 10.1016/j.actatropica.2021.106129
Nilton Barnabé Rodrigues 1 , Luís Eduardo Martinez Villegas 1 , Ana Paula Marques Duarte 2 , Alessandra Silva Orfanó 1 , Breno Dos Anjos Costa 1 , Rafael Nacif Pimenta 1 , Marcus V G Lacerda 3 , Wuelton M Monteiro 2 , Nágila Francinete Costa Secundino 4 , Paulo Filemon Paolucci Pimenta 5
Affiliation  

Anopheles aquasalis is an important malaria vector in coastal regions of South America and islands of the Caribbean. In its original description, the species was divided into two varieties, based on the scaling patterns of their hind-tarsomere 2. Specimens from our 25-year established colony, used for Plasmodium experimental infections, still exhibit both scaling tarsomere patterns. This study examined the DNA sequence of the nuclear Internal Transcribed Spacer 2 (ITS2) and susceptibility to Plasmodium, looking for differences among the phenotypes 30BS and 50BS. One hundred mosquitoes, 25 males and 25 females of each sex, and phenotype were analyzed. Twenty-seven novel haplotypes were identified. Three were found in both phenotypes (30BS and 50BS) regardless of gender. Among the other 27 genotypes, we observed a male-oriented bias in both phenotypic categories. Evaluation of Plasmodium yoelii N67 infections, based on oocyst counts, showed a higher susceptibility of 30BS compared with 50BS. Future studies need to be conducted to evaluate if these genotype assortments among the phenotypic groups reflect differences in fitness, mating, and their susceptibility to infection by Plasmodium parasites.



中文翻译:

在按蚊 (Nyssorhynchus) aquasalis(双翅目:Culicidae)长期群落中个体的表型特征对疟原虫表现出不同的易感性,并表明存在神秘的物种形成

Anopheles aquasalis是南美洲沿海地区和加勒比海岛屿的重要疟疾媒介。在其最初的描述中,该物种根据其后跗骨 2 的鳞片模式分为两个变种。来自我们 25 年建立的殖民地的标本,用于疟原虫实验感染,仍然表现出两种鳞片模式。这项研究检查了核内部转录间隔区 2 (ITS2) 的 DNA 序列和对疟原虫的敏感性,寻找表型 30BS 和 50BS 之间的差异。分析了 100 只蚊子,每种性别 25 只雄性和 25 只雌性,以及表型。确定了 27 个新的单倍型。无论性别如何,在两种表型(30BS 和 50BS)中都发现了三个。在其他 27 种基因型中,我们在两个表型类别中都观察到了面向男性的偏见。根据卵囊计数对约氏疟原虫N67 感染的评估显示,30BS 比 50BS 具有更高的易感性。未来的研究需要进行评估,以评估表型组之间的这些基因型分类是否反映了健康、交配及其对疟原虫寄生虫感染的易感性的差异。

更新日期:2021-09-21
down
wechat
bug