Glatiramer acetate (GA), FDA-approved therapeutic for multiple sclerosis (MS), is a random-sized synthetic polypeptide composed of four amino acids: tyrosine, glutamic acid, alanine, and lysine. This work aims to design an efficient protocol for the separation of GA from its broth. For this purpose, the feasibility of a carbohydrate-based aqueous two-phase system (ATPS) for separation of GA from the amino acids was investigated. Initially, the binodal curve and tie-lines for the ATPS composed of maltodextrin and acetonitrile were determined. Next, preliminary screening experiments for determining optimum conditions were conducted by introducing GA and amino acids into the ATPS individually and the effect of pH and carbohydrate concentration on partition coefficient were investigated. Results revealed that glutamic acid and alanine were migrated toward the acetonitrile rich phase while GA, tyrosine, and lysin showed the opposite trend. Besides, the partitioning of amino acids was correlated with their physicochemical and structural properties. The preliminary results suggested that the system composed of maltodextrin 15 wt%, acetonitrile 35 wt%, at pH = 6 can be considered as the optimum feed. In the next step, the partitioning of GA and amino acids broth was investigated in the optimum feed. Under these conditions, the partition coefficient and selectivity of GA were obtained to be 1.17 and 62%, respectively. Additionally, circular dichroism spectroscopy results proved that the structure of GA remains unchanged during the separation steps. Finally, a recovery method was proposed where the acetonitrile was evaporated and maltodextrin was precipitated by the addition of methanol.

醋酸格拉替雷 (GA) 是 FDA 批准的多发性硬化症 (MS) 治疗剂，是一种随机大小的合成多肽，由四种氨基酸组成：酪氨酸、谷氨酸、丙氨酸和赖氨酸。这项工作旨在设计一种将 GA 与其肉汤分离的有效方案。为此，研究了基于碳水化合物的水性两相系统 (ATPS) 从氨基酸中分离 GA 的可行性。最初，确定了由麦芽糖糊精和乙腈组成的 ATPS 的双节点曲线和连接线。接下来，通过将 GA 和氨基酸分别引入 ATPS 来进行确定最佳条件的初步筛选实验，并研究了 pH 值和碳水化合物浓度对分配系数的影响。结果表明，谷氨酸和丙氨酸向富含乙腈的相迁移，而 GA、酪氨酸和赖氨酸显示出相反的趋势。此外，氨基酸的分配与其理化和结构特性相关。初步结果表明，由麦芽糊精 15 wt%、乙腈 35 wt%、pH = 6 组成的体系可被认为是最佳进料。在下一步中，研究了最佳饲料中 GA 和氨基酸肉汤的分配。在这些条件下，GA 的分配系数和选择性分别为 1.17% 和 62%。此外，圆二色光谱结果证明 GA 的结构在分离步骤中保持不变。最后，