当前位置: X-MOL 学术Biosci. Biotechol. Biochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Low-molecular-weight whey proteins promote collagen production in dermal fibroblasts via the TGF-β receptor/Smad pathway.
Bioscience, Biotechnology, and Biochemistry ( IF 1.6 ) Pub Date : 2021-10-21 , DOI: 10.1093/bbb/zbab155
Takeshi Katayoshi 1 , Yuri Kusano 2 , Takahiro Shibata 3 , Koji Uchida 4 , Kentaro Tsuji-Naito 1
Affiliation  

Whey proteins (WPs) reportedly enhance cutaneous tissue regeneration in in vivo studies. However, the underlying mechanisms of such regenerative processes are poorly understood. In this study, we show that low-molecular-weight WPs (LMWPs; 1-30 kDa) accelerate the dermal collagen production via the transforming growth factor β receptor (TβR)/Smad pathway. We showed that LMWPs increased type I and III collagen expression in normal human dermal fibroblasts. Moreover, LMWPs rapidly induced Smad protein phosphorylation and nuclear translocation. Notably, type I TβR/Smad signaling inhibitor treatment or type II TβR siRNA knockdown blocked the LMWP-induced type I collagen expression. To identify the active components, we fractionated LMWPs and identified β-lactoglobulin and α-lactalbumin as potential TβR/Smad signaling inducers. Our findings unravel novel biological functions of WPs, involving the TβR/Smad-dependent induction of dermal collagen synthesis, highlighting the therapeutic potential of LMWPs in wound healing.

中文翻译:

低分子量乳清蛋白通过 TGF-β 受体/Smad 通路促进真皮成纤维细胞中胶原蛋白的产生。

据报道,乳清蛋白 (WP) 在体内研究中可增强皮肤组织再生。然而,人们对这种再生过程的潜在机制知之甚少。在这项研究中,我们表明低分子量 WP(LMWP;1-30 kDa)通过转化生长因子 β 受体(TβR)/Smad 途径加速真皮胶原蛋白的产生。我们发现 LMWPs 增加了正常人真皮成纤维细胞中 I 型和 III 型胶原蛋白的表达。此外,LMWPs 迅速诱导 Smad 蛋白磷酸化和核转位。值得注意的是,I 型 TβR/Smad 信号抑制剂治疗或 II 型 TβR siRNA 敲低阻断了 LMWP 诱导的 I 型胶原蛋白表达。为了鉴定活性成分,我们对 LMWP 进行分级分离,并将 β-乳球蛋白和 α-乳白蛋白鉴定为潜在的 TβR/Smad 信号传导诱导剂。
更新日期:2021-09-08
down
wechat
bug