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Methanotroph community structure and processes in an inland river affected by natural gas macro-seeps
FEMS Microbiology Ecology ( IF 4.2 ) Pub Date : 2021-09-09 , DOI: 10.1093/femsec/fiab130
Ryan M Burrows 1, 2 , Jodie van de Kamp 3 , Levente Bodrossy 3 , Michael Venarsky 2 , Jack Coates-Marnane 2, 4 , Gavin Rees 5 , Paavo Jumppanen 3 , Mark J Kennard 2
Affiliation  

Methane availability in freshwaters is usually associated with spatial-temporal variation in methanogenesis. Unusually, however, natural gas macro-seeps occur along the Condamine River in eastern Australia which elevate ambient water-column methane concentrations more than 3,000 times. We quantified the spatial-temporal variation in methane oxidation rates and the total microbial and methanotroph community composition (through the amplification and sequencing of 16S rRNA and particulate methane monooxygenase (pmoA) genes), and the factors mediating this variation, in reaches with and without macro-seeps. Sediment methane oxidation rates were, on average, 29 times greater, and the abundance of methanotrophs significantly higher, in the vicinity of methane macro-seeps compared to non-seep sites. Methylocystis was the most abundant methanotroph group at all sites, but type Ib methanotrophs showed the steepest increase in abundance at seep sites. pmoA gene analysis identified these as clade 501, while 16S rRNA gene analysis identified these as the closely related genus Methylocaldum. Sediment methane oxidation rates and the relative abundance and composition of benthic microbial communities were primarily influenced by methane availability which was in turn related to variation in river discharge. Methane-derived carbon may be an important energy source for the aquatic food webs in reaches affected by natural gas macro-seeps.

中文翻译:

受天然气宏观渗漏影响的内河甲烷氧化菌群落结构与过程

淡水中的甲烷可用性通常与甲烷生成的时空变化有关。然而,不同寻常的是,沿着澳大利亚东部康达明河发生天然气大渗漏,使周围水柱中的甲烷浓度升高了 3,000 多倍。我们量化了甲烷氧化速率和总微生物和甲烷氧化菌群落组成的时空变化(通过 16S rRNA 和颗粒甲烷单加氧酶 ( pmoA ) 基因的扩增和测序),以及介导这种变化的因素,有和没有宏观渗漏。与非渗漏地点相比,甲烷大渗漏附近的沉积物甲烷氧化率平均高 29 倍,甲烷氧化菌的丰度明显更高。Methylocystis是所有地点最丰富的甲烷氧化菌组,但 Ib 型甲烷氧化菌在渗漏地点的丰度增加幅度最大。pmo A 基因分析将这些鉴定为进化枝 501,而 16S rRNA 基因分析将它们鉴定为密切相关的Methylocaldum属。沉积物甲烷氧化速率和底栖微生物群落的相对丰度和组成主要受甲烷可用性的影响,而甲烷可用性又与河流流量的变化有关。甲烷衍生的碳可能是受天然气宏观渗漏影响的河段中水生食物网的重要能源。
更新日期:2021-10-09
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