Stem cell responses in tissues after exposure to radiation are of significance for maintaining tissue functions. From the point of view of stem cell characteristics, this article seeks to illustrate some contributions of microbeam research to spatially fractionated radiotherapy (SFRT), such as grid radiotherapy and microbeam radiotherapy. Although the tissue-sparing response after SFRT was first reported more than a century ago, current radiation dose–volume metrics are still unable to accurately predict such tissue-level changes in response to spatially fractionated radiation fields. However, microbeam approaches could contribute to uncovering the mechanisms of tissue response, significantly improving the outcomes of SFRT and reducing its adverse effects. Studies with microbeams have shown that the testicular tissue-sparing effect for maintaining spermatogenesis after exposure to spatially fractionated radiation depends on biological parameters, such as the radiation dose distribution at the microscale level for tissue-specific stem cells and the microenvironment, or niche. This indicates that stem cell survival, migration, and repopulation are involved in the tissue-level changes during or after SFRT. The illustration of microbeam applications in this article focuses on the stem cell migration as a possible mechanism of the tissue-sparing effect for preserving functionality.

暴露于辐射后组织中的干细胞反应对于维持组织功能具有重要意义。本文试图从干细胞特性的角度来说明微束研究对空间分割放射治疗（SFRT）的一些贡献，例如网格放射治疗和微束放射治疗。尽管 SFRT 后的组织保留反应在一个多世纪前首次被报道，但当前的辐射剂量-体积指标仍然无法准确预测响应空间分割辐射场的这种组织水平变化。然而，微束方法有助于揭示组织反应的机制，显着改善 SFRT 的结果并减少其不利影响。微束研究表明，暴露于空间分割辐射后维持精子发生的睾丸组织保留效应取决于生物学参数，例如组织特异性干细胞和微环境或生态位的微尺度辐射剂量分布。这表明干细胞存活、迁移和再增殖与 SFRT 期间或之后的组织水平变化有关。本文中微束应用的说明侧重于干细胞迁移，作为保留功能的组织保留效应的一种可能机制。或利基。这表明干细胞存活、迁移和再增殖与 SFRT 期间或之后的组织水平变化有关。本文中微束应用的说明侧重于干细胞迁移，作为保留功能的组织保留效应的一种可能机制。或利基。这表明干细胞存活、迁移和再增殖与 SFRT 期间或之后的组织水平变化有关。本文中微束应用的说明侧重于干细胞迁移，作为保留功能的组织保留效应的一种可能机制。