当前位置: X-MOL 学术Carbohydr. Polym. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A thermo-sensitive chitosan/pectin hydrogel for long-term tumor spheroid culture
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2021-09-09 , DOI: 10.1016/j.carbpol.2021.118633
Giulia Morello 1 , Alessandra Quarta 2 , Antonio Gaballo 2 , Lorenzo Moroni 3 , Giuseppe Gigli 1 , Alessandro Polini 2 , Francesca Gervaso 2
Affiliation  

Hydrogels represent a key element in the development of in vitro tumor models, by mimicking the typical 3D tumor architecture in a physicochemical manner and allowing the study of tumor mechanisms. Here we developed a thermo-sensitive, natural polymer-based hydrogel, where chitosan and pectin were mixed and, after a weak base-induced chitosan gelation, a stable semi-Interpenetrating Polymer Network formed. This resulted thermo-responsive at 37 °C, injectable at room temperature, stable up to 6 weeks in vitro, permeable to small/medium-sized molecules (3 to 70 kDa) and suitable for cell-encapsulation. Tunable mechanical and permeability properties were obtained by varying the polymer content. Optimized formulations successfully supported the formation and growth of human colorectal cancer spheroids up to 44 days of culture. The spheroid dimension and density were influenced by the semi-IPN stiffness and permeability. These encouraging results would allow the implementation of faithful tumor models for the study and development of personalized oncological treatments.



中文翻译:

用于长期肿瘤球体培养的热敏壳聚糖/果胶水凝胶

通过以物理化学方式模拟典型的 3D 肿瘤结构并允许研究肿瘤机制,水凝胶代表了体外肿瘤模型开发的关键要素。在这里,我们开发了一种热敏、天然聚合物基水凝胶,其中壳聚糖和果胶混合,在弱碱诱导的壳聚糖凝胶化后,形成了稳定的半互穿聚合物网络。这导致在 37 °C 下具有热响应性,在室温下可注射,在体外稳定长达 6 周,可渗透小/中型分子(3 至 70 kDa),适用于细胞封装。通过改变聚合物含量获得了可调的机械和渗透性能。优化的配方成功地支持人类结直肠癌球体的形成和生长长达 44 天的培养。球体尺寸和密度受半 IPN 刚度和渗透性的影响。这些令人鼓舞的结果将允许实施可靠的肿瘤模型来研究和开发个性化的肿瘤治疗。

更新日期:2021-09-16
down
wechat
bug