Lignocellulosic biomass-derived nanocellulose has been attracting more and more attentions due to its distinguished advantages and various applications, but its development has been restricted by the preparation especially with environmental friendly approach. Herein, lignin-containing cellulose nanofibrils (LCNF) was prepared from corncob via the combined pretreatment of choline chloride-based DES (ChCl-DES) and enzymatic hydrolysis followed by high-pressure homogenization. The effects of different types of ChCl-DES on the properties of LCNF were investigated and compared. The results showed that LCNF can be successfully fabricated through the combined pretreatments; the LCNF had an average diameter of 60–90 nm, exhibited good fluorescence, high thermal stability (up to 353 °C of T_max), hydrophobicity, stability, and redispersibility in organic solvent; AC-LCNF showed well oriented arrangement, the highest hydrophobicity and fluorescence, and distinguished redispersibility especially in DMSO. ChCl-DES as one green and sustainable approach would realize efficient separation and high value-added utilization of agricultural residues.

木质纤维素生物质衍生的纳米纤维素因其显着的优势和广泛的应用而受到越来越多的关注，但其发展受到制备尤其是环保方法的限制。在此，含木质素的纤维素纳米纤丝 (LCNF) 是由玉米芯通过氯化胆碱基 DES (ChCl-DES) 和酶水解的联合预处理和高压均质化制备的。研究并比较了不同类型的 ChCl-DES 对 LCNF 性质的影响。结果表明，通过联合预处理可以成功制备LCNF；LCNF 的平均直径为 60-90 nm，表现出良好的荧光性，高热稳定性（高达 353 °C 的 T _max)、疏水性、稳定性和在有机溶剂中的再分散性；AC-LCNF 显示出良好的定向排列、最高的疏水性和荧光性以及卓越的再分散性，尤其是在 DMSO 中。ChCl-DES 作为一种绿色和可持续的方法，将实现农业残留物的高效分离和高附加值利用。