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Pegylated Eu-enabled submicron alumina spheres as potential theranostics agent RD cell line as model
Saudi Journal of Biological Sciences ( IF 4.4 ) Pub Date : 2021-09-08 , DOI: 10.1016/j.sjbs.2021.09.001
Numrah Sultan 1 , Syed Mujtaba Ul Hassan 2 , Ahmat Khurshid 3 , M Fakhar-E-Alam 4 , Faisal Shahzad 2 , Attaullah Shah 5 , Muhammad Atif 6 , Shafiq Ahmad 7 , Muhammad Tamoor Masood 8
Affiliation  

Objectives

This study is aimed to synthesis and evaluate PEGylated Eu enabled spherical alumina submicron particles (s-Al2O3:Eu) for potential theranostic applications.

Methods

This study is bisected into two parts, a) synthesis of PEGylated Eu enabled spherical alumina submicron particles (s-Al2O3:Eu), and b) characterization of the synthesized particles to determine their efficacy for potential theranostic applications.

The synthesis of the particles involved the following steps. In the first step, s-Al2O3:Eu is synthesized using solvothermal synthesis. In the next step, the particles undergo post synthesis water–ethanol treatment and calcination. The surface of the synthesized s-Al2O3:Eu particles is then coated by PEG to increase its biocompatibility.

Once the particles are prepared, they are characterized using different techniques. The microstructure, composition and structure of the particles is characterized using SEM, EDX and XRD techniques. The detection of the functional groups is done using FTIR analysis. The photoluminescence emission spectrum of s-Al2O3:Eu is studied using Photoluminescence spectroscopy. And, finally, the biocompatibility is studied using MTT assay on RD cell lines.

Results

The microstructure analysis, from the micrographs obtained from SEM, shows that the spherical alumina particles have a submicron size with narrow size distribution. The compositional analysis, as per EDX, confirms the presence of Oxygen, Aluminum and Europium in the particles. While, XRD analysis of s-Al2O3:Eu confirms the formation of alpha alumina phase after calcination at 700 °C. Emission peaks, obtained by Photoluminescence emission spectroscopy, show that the optimum emission intensities correspond to the transition from 5D0 to 7Fj orbital of Eu+3. FTIR analysis confirms the successful coating of PEG. Finally, a cell viability of more than 86% is observed when the biocompatibility of the particles is studied, using MTT assay on RD cell lines.

Conclusions

s-Al2O3:Eu with narrow distribution are successfully synthesized. Structural and functional characterizations support the suitability of s-Al2O3:Eu as potential theranostic agent.



中文翻译:

聚乙二醇化 Eu 亚微米氧化铝球作为潜在的治疗剂 RD 细胞系作为模型

目标

本研究旨在合成和评估聚乙二醇化 Eu 启用的球形氧化铝亚微米颗粒 (s-Al 2 O 3 :Eu) 用于潜在的治疗应用。

方法

该研究分为两部分,a) 聚乙二醇化 Eu 启用的球形氧化铝亚微米颗粒 (s-Al 2 O 3 :Eu) 的合成,和 b) 合成颗粒的表征以确定它们对潜在治疗应用的功效。

粒子的合成涉及以下步骤。第一步,使用溶剂热合成法合成s-Al 2 O 3 :Eu。在下一步中,颗粒经过合成后的水-乙醇处理和煅烧。合成的 s-Al 2 O 3 :Eu 颗粒的表面随后被 PEG 包覆以增加其生物相容性。

一旦制备了颗粒,就使用不同的技术对其进行表征。使用 SEM、EDX 和 XRD 技术表征颗粒的微观结构、组成和结构。官能团的检测是使用 FTIR 分析完成的。使用光致发光光谱研究了s-Al 2 O 3 :Eu的光致发光发射光谱。最后,使用 MTT 法对 RD 细胞系进行生物相容性研究。

结果

由 SEM 获得的显微照片的微观结构分析表明,球形氧化铝颗粒具有亚微米尺寸,尺寸分布窄。根据 EDX,成分分析证实颗粒中存在氧、铝和铕。同时,s-Al 2 O 3 :Eu 的 XRD 分析证实在 700 °C 煅烧后形成了 α 氧化铝相。通过光致发光发射光谱获得的发射峰表明,最佳发射强度对应于Eu +3从5 D 07 F j轨道的跃迁. FTIR 分析证实了 PEG 的成功包被。最后,在 RD 细胞系上使用 MTT 分析研究颗粒的生物相容性时,观察到超过 86% 的细胞活力。

结论

成功合成了分布窄的s-Al 2 O 3 :Eu。结构和功能表征支持 s-Al 2 O 3 :Eu 作为潜在的治疗剂的适用性。

更新日期:2021-10-27
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