Twelve guaianolide-type sesquiterpene oligomers with diverse structures were isolated from the whole plants of Ainsliaea fragrans, including a novel trimer (1) and two new dimers (2, 3). The chemical structures of the new compounds were elucidated through spectroscopic data interpretation and computational calculations. Ainsfragolide (1) is an unusual guaianolide sesquiterpene trimer generated with a novel C–C linkage at C_2’–C_15″, which may be biosynthesized prospectively through a further Michael addition. Cytotoxicity results showed that ainsfragolide (1) was the most potent compound against five cancer cell lines with IC₅₀ values in the range of 0.4–8.3 μM.

中文翻译：

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来自 Ainsliaea fragrans 的细胞毒性愈创木内酯倍半萜类化合物

具有不同结构十二guaianolide型倍半萜烯低聚物是从整个植物中分离兔儿风香，包括一种新颖的三聚体（1）和两个新的二聚体（2，3）。通过光谱数据解释和计算计算阐明了新化合物的化学结构。Ainsfragolide ( 1 ) 是一种不寻常的愈创木酚内酯倍半萜烯三聚体，在 C _2' -C _15"处具有新的 C-C 键，可以通过进一步的迈克尔加成进行前瞻性生物合成。细胞毒性结果表明黄芪甲苷 ( 1 ) 是对五种具有 IC 的癌细胞系最有效的化合物。0.4–8.3 μM 范围内的_{50 个}值。