Glycans have been hot topics in recent years due to their exhibition of numerous biological activities. However, the heterogeneity of their natural source and the complexity of their chemical synthesis impede the progress in their biological research. Thus, the development of glycan assembly strategies to acquire plenty of structurally well-defined glycans is an important issue in carbohydrate chemistry. In this review, the latest advances in glycan assembly strategies from concepts to their applications in carbohydrate synthesis, including chemical and enzymatic/chemo-enzymatic approaches, as well as solution-phase and solid-phase/tag-assisted synthesis, are summarized. Furthermore, the automated glycan assembly techniques are also outlined.

中文翻译：

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聚糖组装策略：从概念到应用

聚糖由于表现出众多的生物活性而成为近年来的热门话题。然而，其天然来源的异质性和化学合成的复杂性阻碍了其生物学研究的进展。因此，开发聚糖组装策略以获取大量结构明确的聚糖是碳水化合物化学中的一个重要问题。在这篇综述中，总结了聚糖组装策略的最新进展，从概念到它们在碳水化合物合成中的应用，包括化学和酶促/化学酶促方法，以及液相和固相/标签辅助合成。此外，还概述了自动聚糖组装技术。