After a decade of experimental applications, it is the objective of this review to make a point on combinatorial peptide ligand libraries dedicated to low-abundance proteins from animals to plants and to microorganism proteomics. It is, thus, at the light of the recent technical developments and applications that we will examine the state of the art, its usage within the scientific community, and its openness to unexplored fields. The improvements of the methodology and its implementation in connection with analytical determinations of combinatorial peptide ligand library (CPLL)-treated samples are extensively reviewed and commented upon. Relevant examples covering few critical aspects describe the performance of the technology. Finally, a reflection on the technological future is attempted in particular by involving new concepts adapted to the limited availability of certain biological samples.

中文翻译：

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组合肽：持续探测低丰度蛋白质的文库

经过十年的实验应用，本综述的目的是对专用于从动物到植物和微生物蛋白质组学的低丰度蛋白质的组合肽配体库提出观点。因此，鉴于最近的技术发展和应用，我们将检查最先进的技术、它在科学界的使用以及它对未开发领域的开放性。该方法的改进及其与组合肽配体库 (CPLL) 处理样品的分析测定相关的实施得到了广泛的审查和评论。涵盖几个关键方面的相关示例描述了该技术的性能。最后，