Novel resistance sources to the pathogen Puccinia striiformis f. sp. tritici, which causes yellow rust (stripe rust), a widespread devastating foliar disease in wheat (Triticum aestivum L.), are in demand. Here, we tested two doubled haploid (DH) spring wheat populations derived from the genetic resources for resistance to yellow rust in field trials in Germany and Egypt. Additionally, we performed tests for all-stage resistance (seedling resistance). We performed linkage mapping based on 15k Infinium SNP chip genotyping data that resulted in 3,567 and 3,457 polymorphic markers for DH Population 1 (103 genotypes) and DH Population 2 (148 genotypes), respectively. In DH Population 1, we identified a major and consistent quantitative trait locus (QTL) on chromosome 1B that explained up to 28 and 39% of the phenotypic variation in the field and seedling tests, respectively. The favorable allele was contributed by the line ‘TRI-5645’, a landrace from Iran, and is most probably the yellow rust resistance (Yr) gene Yr10. In DH Population 2, the favorable allele of a major QTL on chromosome 6B was contributed by the line ‘TRI-5310’, representing the variety ‘Eureke’ from France. This QTL was mainly effective in the German environments and explained up to 36% of the phenotypic variation. In Egypt, however, only a moderate resistance QTL was identified in the field tests and no resistance QTL was observed in the seedling tests. Our results demonstrate the usefulness of genetic resources to identify novel sources of resistance to yellow rust, including the “Warrior” race PstS10.

中文翻译：

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德国联邦异地基因库遗传资源开发的春小麦双单倍体群体抗黄锈病数量性状位点

对病原体Puccinia striiformis f 的新抗性来源。sp。小麦，它会导致黄锈病（条锈病），这是小麦（Triticum aestivum ）中广泛存在的破坏性叶面病害L.), 需求量很大。在这里，我们在德国和埃及的田间试验中测试了来自遗传资源的两个双单倍体 (DH) 春小麦种群的抗黄锈病。此外，我们还进行了全阶段抗性（幼苗抗性）测试。我们基于 15k Infinium SNP 芯片基因分型数据进行连锁作图，分别为 DH 种群 1（103 个基因型）和 DH 种群 2（148 个基因型）产生了 3,567 和 3,457 个多态性标记。在 DH 种群 1 中，我们在 1B 号染色体上确定了一个主要且一致的数量性状基因座 (QTL)，分别解释了田间和幼苗试验中高达 28% 和 39% 的表型变异。有利的等位基因来自伊朗的地方品种“TRI-5645”，很可能是抗黄锈病（Yr) 基因Yr10。在 DH 种群 2 中，染色体 6B 上一个主要 QTL 的有利等位基因由代表法国品种“Eureke”的“TRI-5310”系提供。该 QTL 主要在德国环境中有效，并解释了高达 36% 的表型变异。然而，在埃及，在田间试验中仅鉴定出中等抗性QTL，而在幼苗试验中未观察到抗性QTL。我们的研究结果证明了遗传资源在鉴定新的黄锈病抗性来源方面的有用性，包括“勇士”种族PstS10。