Phase transition is a promising aspect of DNA as biopolymers. Anionic DNA oligonucleotides easily form complexes with cationic polypeptides such as polylysine, and duplex formation significantly influences their complexation and resulting microcompartments. In this study, phase transition of microcompartments containing DNA and polylysine was systematically induced by modulating duplex formation of arylazopyrazole-conjugated oligonucleotides with light. We demonstrated that UV irradiation destabilized DNA duplex and generated isotropic coacervates, while duplex stabilization by visible light irradiation caused the formation of liquid crystalline coacervates. This photocontrol of phase transition was highly repeatable, and similar changes were observed even after ten cycles of light irradiation. Our approach would provide a robust control layer to the development of tailor-made microcompartments.

中文翻译：

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芳基并吡唑共轭寡核苷酸的光控相变

相变是 DNA 作为生物聚合物的一个有前途的方面。阴离子 DNA 寡核苷酸很容易与阳离子多肽（如聚赖氨酸）形成复合物，双链体的形成会显着影响它们的复合和由此产生的微区室。在这项研究中，通过用光调节芳基并吡唑偶联的寡核苷酸的双链体形成，系统地诱导了含有 DNA 和聚赖氨酸的微区室的相变。我们证明紫外线照射使 DNA 双链不稳定并产生各向同性凝聚层，而可见光照射的双链稳定导致液晶凝聚层的形成。这种相变的光控制是高度可重复的，即使在十次光照射循环后也观察到类似的变化。