The vitamin B₁₂ content of lobster, crayfish, crab, and shrimp viscera was determined using a microbiological assay based on Lactobacillus delbrueckii subsp. lactis ATCC7830. These viscera contained high vitamin B₁₂ levels (7.2–118.6 μg/100 g of wet weight). We purified vitamin B₁₂ compounds from the extracts of these viscera using an immunoaffinity column. Corrinoid compounds were identified using liquid chromatography-electrospray ionization tandem mass spectrometry. The viscera tested contained vitamin B₁₂-b-, -d-, and -e-monocarboxylic acids and vitamin B₁₂-be-dicarboxylic acid, modified with peripheral propionamide side chains of the corrin ring. These vitamin B₁₂ carboxylic acids are specifically located in the viscera edible portions of lobster, crayfish, crab, and shrimp.

中文翻译：

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龙虾、小龙虾、螃蟹和虾的内脏可食用部分中维生素 B12 羧酸的表征

龙虾、小龙虾、螃蟹和虾内脏的维生素 B ₁₂含量使用基于德氏乳杆菌亚种的微生物测定法测定。乳酸ATCC7830。这些内脏含有高水平的维生素 B ₁₂（7.2–118.6 μg/100 g 湿重）。我们使用免疫亲和柱从这些内脏的提取物中纯化了维生素 B ₁₂化合物。使用液相色谱-电喷雾电离串联质谱法鉴定类可啉化合物。测试的内脏含有维生素 B ₁₂ - b -、- d - 和 - e -单羧酸和维生素 B ₁₂ - be-二羧酸，用corrin环的外围丙酰胺侧链修饰。这些维生素 B ₁₂羧酸特别位于龙虾、小龙虾、螃蟹和虾的内脏可食用部分。