The rapid spread of viral infections demands early detection strategies to minimize proliferation of the disease. Here, we demonstrate a plasmonic biosensor to detect Dengue virus, which was chosen as a model, via its nonstructural protein NS1 biomarker. The sensor is functionalized with a synthetic single-stranded DNA oligonucleotide and provides high affinity toward NS1 protein present in the virus genome. We demonstrate the detection of NS1 protein at a concentration of 0.1–10 μg/mL in bovine blood using an on-chip microfluidic plasma separator integrated with the plasmonic sensor which covers the clinical threshold of 0.6 μg/mL of high risk of developing Dengue hemorrhagic fever. The conceptual and practical demonstration shows the translation feasibility of these microfluidic optical biosensors for early detection of a wide range of viral infections, providing a rapid clinical diagnosis of infectious diseases directly from minimally processed biological samples at point of care locations.

中文翻译：

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用于直接检测血液中病毒生物标志物的 DNA 修饰等离子体传感器

病毒感染的快速传播需要早期检测策略以最大限度地减少疾病的扩散。在这里，我们展示了一种等离子体生物传感器，通过其非结构蛋白 NS1 生物标志物来检测被选为模型的登革热病毒。该传感器使用合成的单链 DNA 寡核苷酸进行功能化，并提供对病毒基因组中存在的 NS1 蛋白的高亲和力。我们证明了使用与等离子体传感器集成的片上微流体血浆分离器检测牛血中浓度为 0.1-10 μg/mL 的 NS1 蛋白，该传感器涵盖了 0.6 μg/mL 的登革热出血高风险临床阈值。发烧。