In living organisms, tyrosinase selectively produces l-DOPA from l-tyrosine. Here, a bicomponent hydrogel is used as a template for tyrosinase-catalyzed selective generation of l-DOPA from tyrosine. An amphiphilic molecule 1,5-diaminonaphthalene (DAN) coassembles with 1,3,5-benzenetricarboxylic acid (BTC) to form a self-supporting hydrogel. After alteration of complementary acids, DAN does not coassemble to form a hydrogel. The coassembly mechanism is investigated using spectroscopic techniques. The transmission electron microscopy and scanning electron microscopy images reveal the morphology details. The l-DOPA is kept from being oxidized when the hydrogel is used as a template. The enzymatically synthesized l-DOPA can also be separated from the mixture by easy tuning of the bicomponent coassembly.

中文翻译：

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双组分共组装水凝胶作为多巴选择性酶促生成的模板

在生物体中，酪氨酸酶从l-酪氨酸选择性地产生l- DOPA 。在这里，双组分水凝胶用作酪氨酸酶催化从酪氨酸选择性生成l-多巴的模板。两亲分子 1,5-二氨基萘 ( DAN ) 与 1,3,5-苯三羧酸 ( BTC )共组装形成自支撑水凝胶。互补酸改变后，DAN不会共同组装形成水凝胶。使用光谱技术研究了共组装机制。透射电子显微镜和扫描电子显微镜图像揭示了形态细节。该升当水凝胶用作模板时，-DOPA 不会被氧化。酶促合成的l- DOPA 也可以通过双组分共组装的简单调整从混合物中分离出来。