Antibody mimetics are proteins smaller than antibodies that have antigen-binding properties. Here, we used a combination of informatic selection and experimental verification to identify from a public database several candidate scaffold proteins. From those candidates, we selected a protein with high thermal stability, bacterial expression, and mutation tolerance and used molecular engineering and phage display selection techniques to develop the scaffold into an antibody mimetic with binding affinity for galactin-3, a biomarker of cancer and heart disease.

中文翻译：

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选择用于开发作为抗体模拟物的支架蛋白的组合信息学和实验方法

抗体模拟物是比具有抗原结合特性的抗体更小的蛋白质。在这里，我们使用信息选择和实验验证的组合从公共数据库中识别出几种候选支架蛋白。从这些候选物中，我们选择了一种具有高热稳定性、细菌表达和突变耐受性的蛋白质，并使用分子工程和噬菌体展示选择技术将支架开发成对 galactin-3（癌症和心脏的生物标志物）具有结合亲和力的抗体模拟物疾病。