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Lactobacillus rhamnosus GG Promotes Early B Lineage Development and IgA Production in the Lamina Propria in Piglets
The Journal of Immunology ( IF 4.4 ) Pub Date : 2021-10-15 , DOI: 10.4049/jimmunol.2100102
Yu-Bei Jin 1, 2 , Xin Cao 1 , Chun-Wei Shi 1 , Bo Feng 1 , Hai-Bin Huang 1 , Yan-Long Jiang 1 , Jian-Zhong Wang 1 , Gui-Lian Yang 3 , Wen-Tao Yang 3 , Chun-Feng Wang 3
Affiliation  

Gut microbes play an important role in the development of host B cells. It has been controversial whether GALT is the development site of B cells in pigs. By investigating the relationship between gut microbes and the development of B cells in the GALT of piglets, we found, to our knowledge for the first time, that early B cells exist in the gut lamina propria (LP) in pigs at different ages. We further used Lactobacillus rhamnosus GG (LGG) to treat piglets. The results showed that LGG promotes the development of the early B lineage, affects the composition of the Ig CDR3 repertoires of B cells, and promotes the production of IgA in the intestinal LP. Additionally, we found that the p40 protein derived from LGG can activate the EGFR/AKT and NF-κB signaling pathways, inducing porcine intestinal epithelial cells (IPEC-J2) to secrete a proliferation-inducing ligand (APRIL), which promotes IgA production in B cells. Finally, we identified ARF4 and DIF3 as candidates for p40 receptors on IPEC-J2 by GST pull-down, liquid chromatography-mass spectrometry/mass spectrometry analysis, and coimmunoprecipitation. In conclusion, LGG could promote early B cell differentiation and development in the intestinal LP in piglets and might contribute to promoting IgA production via secretion of p40, which interacts with the membrane receptors on IPEC-J2 and induces them to secrete APRIL. Our study will provide insight to aid in better utilization of probiotics to increase human health.



中文翻译:

鼠李糖乳杆菌GG促进仔猪固有层早期B谱系发育和IgA产生

肠道微生物在宿主 B 细胞的发育中起重要作用。GALT是否是猪B细胞的发育部位一直存在争议。通过研究肠道微生物与仔猪 GALT 中 B 细胞发育之间的关系,我们首次发现,早期 B 细胞存在于不同年龄猪的肠道固有层 (LP) 中。我们进一步使用了鼠李糖乳杆菌GG (LGG) 治疗仔猪。结果表明,LGG促进早期B谱系的发育,影响B细胞Ig CDR3库的组成,促进肠LP中IgA的产生。此外,我们发现源自 LGG 的 p40 蛋白可以激活 EGFR/AKT 和 NF-κB 信号通路,诱导猪肠上皮细胞 (IPEC-J2) 分泌增殖诱导配体 (APRIL),促进 IgA 的产生。 B细胞。最后,我们通过 GST pull-down、液相色谱-质谱/质谱分析和共免疫沉淀将 ARF4 和 DIF3 鉴定为 IPEC-J2 上 p40 受体的候选物。综上所述,LGG 可以促进仔猪肠道 LP 的早期 B 细胞分化和发育,并可能通过分泌 p40 促进 IgA 的产生,p40 与 IPEC-J2 上的膜受体相互作用并诱导它们分泌 APRIL。我们的研究将为帮助更好地利用益生菌来提高人类健康提供见解。

更新日期:2021-10-06
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