An electrochemical sensing system was proposed for tryptophan (Trp) measurement. CuCo₂O₄@ biomass derived carbon (CuCo₂O₄@BC) was combined with molecularly imprinted polymer (MIP) to construct the electrochemical sensor. The BC was obtained by pyrolyzing leaves of Lactuca sativa L. var. ramosa Hort, and the CuCo₂O₄@BC was synthesized via hydrothermal method. The MIP was electropolymerized on CuCo₂O₄@BC modified glassy carbon electrode (CuCo₂O₄@BC/GCE) using pyrrole as the monomer and Trp as the template. The sensor can specifically rebind Trp because of the specific recognition ability of the MIP. After optimizing the experimental parameters, the differential pulse voltammetry (DPV) current of the fabricated sensor is linearly correlated with Trp concentration in the ranges of 0.01 μM to1 μM and 1 μM to 40 μM, and the limit of detection (LOD) is 0.003 μM. Moreover, the presented sensor holds excellent selectivity toward Trp, and has been successfully applied to detect Trp in human serum and urine with satisfactory recoveries.

提出了一种用于色氨酸 (Trp) 测量的电化学传感系统。CuCo ₂ O ₄ @生物质衍生碳（CuCo ₂ O ₄ @BC）与分子印迹聚合物（MIP）结合以构建电化学传感器。BC 是通过热解Lactuca sativa L. var. 的叶子获得的。ramosa Hort和 CuCo ₂ O ₄ @BC 是通过水热法合成的。MIP 在 CuCo ₂ O ₄ @BC 改性玻碳电极（CuCo ₂ O ₄@BC/GCE) 使用吡咯作为单体和色氨酸作为模板。由于MIP的特定识别能力，传感器可以特异性地重新结合Trp。优化实验参数后，制成的传感器的差分脉冲伏安（DPV）电流与Trp浓度在0.01μM至1μM和1μM至40μM范围内线性相关，检测限（LOD）为0.003μM . 此外，所提出的传感器对 Trp 具有出色的选择性，并已成功应用于检测人血清和尿液中的 Trp，回收率令人满意。