Reaction flow (RF) chromatography is a powerful and efficient approach that utilizes conventional high-performance liquid chromatography (HPLC)–ultraviolet (UV)–visible detection. This technique exploits a novel column end-fitting and an extra HPLC pump that delivers a reagent specific for selective detection, in particular the antioxidant profiling of natural products. This study employed RF for the first time to identify antioxidants in a commercial ginger sample. This demonstrated the previously validated assay's ease and power to extract information about the natural product's antioxidant properties. Due to the simplicity involved with data analysis and peak matching process, the following information was revealed between the chemical and antioxidant profiles: three of the strongest antioxidant activity peaks in the ginger sample (593 nm) did not correlate with the three most abundant chemical profile peaks (UV absorbance at 254 and 280 nm); the ratio of seven antioxidant peaks may be potentially used for food authenticity purposes, and future research should target these peaks for the early discovery of novel antioxidants sourced in ginger. Utilization of this previously validated assay provided the resolution of numerous peaks in the ginger extract and information associated with their antioxidant attributes and chemical abundance. This approach is more informative than total antioxidant assays that lack compound specificity information. Furthermore, it is superior to mass spectrometric (MS) assays that cannot evaluate each compound's antioxidant strength, and does not involve the expense involved in the acquisition and maintenance of the MS detection hardware, and does not require the high level of expertise needed to conduct the MS data analysis.

反应流 (RF) 色谱是一种强大而有效的方法，它利用了传统的高效液相色谱 (HPLC)-紫外 (UV)-可见光检测。该技术利用了一种新型的色谱柱末端接头和一个额外的 HPLC 泵，该泵提供了一种专用于选择性检测的试剂，特别是天然产物的抗氧化剂分析。本研究首次使用 RF 来鉴定商业生姜样品中的抗氧化剂。这证明了先前经过验证的测定方法可以轻松和强大地提取有关天然产品抗氧化特性的信息。由于数据分析和峰匹配过程的简单性，在化学和抗氧化剂特征之间揭示了以下信息：姜样品中三个最强的抗氧化活性峰（593 nm）与三个最丰富的化学谱峰（254 和 280 nm 处的紫外吸收）无关；七个抗氧化剂峰的比例可能用于食品真实性目的，未来的研究应该针对这些峰，以便早期发现源自生姜的新型抗氧化剂。利用这种先前经过验证的测定法可以解析生姜提取物中的众多峰，并提供与其抗氧化特性和化学丰度相关的信息。这种方法比缺乏化合物特异性信息的总抗氧化检测提供更多信息。此外，它优于无法评估每种化合物抗氧化强度的质谱 (MS) 分析，