Abstract

The present study demonstrates the pharmacological tendency of Sideroxylon mascatense leaves extracts, fractions and sub-fractions using thin layer chromatography, column chromatography, and phytochemical (phenolics, flavonoids) and biological assays (free radical scavenging, antioxidative, antimicrobial, enzyme inhibition). The results disclosed that fractionation practice accumulated the active phytochemicals in few fractions and finally leads to the isolation of active compounds. The structural elucidation was carried out using spectroscopic 1D (¹H, ¹³C) 2D NMR and spectrometric techniques. The n-hexane fraction led to isolation of lupeol. From the CHCl₃ and EtOAc fractions, two compounds were isolated, hentriacontanol, and lupeol, respectively. The isolated compounds were also characterized for biological activities. This study concludes that bioactivity guided isolation can be performed for isolation of active constituents from S. mascatense which can be further explored for drug development.

摘要

本研究使用薄层色谱法、柱色谱法、植物化学（酚类、黄酮类化合物）和生物测定法（自由基清除、抗氧化、抗菌、酶抑制）证明了马六甲叶提取物、级分和亚级分的药理趋势。结果表明，分馏实践在几个馏分中积累了活性植物化学物质，并最终导致活性化合物的分离。^{使用光谱 1D ( 1} H, ¹³ C) 2D NMR 和光谱技术进行结构解析。正己烷部分导致羽扇豆醇的分离。从 CHCl ₃和 EtOAc 馏分，分别分离出两种化合物，十六烷醇和羽扇豆醇。分离的化合物也被表征为生物活性。该研究得出结论，可以进行生物活性引导分离以从S. mascatense中分离活性成分，这可以进一步探索药物开发。