Abstract

A new icetexane diterpenoid, 11, 12, 20α-trihydroxyl-7β-methoxyicetexa-8, 11, 13-triene-19, 10-lactone [Phyllane A (1)], and a new abietane diterpenoid, 7β, 20-epoxy-3β, 17-acetoxy-abieta-8, 11, 13-teriene-11, 12-diol [phyllane B (2)], along with two known compounds (3 and 4) were isolated from the methanol (MeOH) extract of twigs and leaves of the folk medicinal Isodon phyllopodus. Their structures were determined by spectroscopic analyses including 2 D NMR spectral data, and further confirmed by X-ray single crystal diffraction. Moreover, the compounds were evaluated for their cytotoxicity and anti-HIV activities, and phyllane A showed anti-HIV activity with an IC₅₀ value of 15.7 μM, but phyllane B was found to be cytotoxic to the A549 host cells with a CC₅₀ value of 108.5 μM.

摘要

一种新的 icetexane 双萜，11, 12, 20α-trihydroxy-7β-methoxyicetexa-8, 11, 13-triene-19, 10-lactone [Phyllane A ( 1 )]，以及一种新的松香二萜，7β, 20-epoxy-从树枝的甲醇 (MeOH) 提取物中分离出3β, 17-acetoxy-abieta-8, 11, 13-teriene-11, 12-diol [phyllane B ( 2 )] 以及两种已知化合物（3和4 ）和民间药用叶氏等齿龙的叶子。它们的结构通过光谱分析确定，包括 2 D NMR 光谱数据，并通过 X 射线单晶衍射进一步证实。此外，还评估了这些化合物的细胞毒性和抗 HIV 活性，phyllane A 显示出抗 HIV 活性，IC_{为 50}值为 15.7 μM，但发现叶烷 B 对 A549 宿主细胞具有细胞毒性，CC ₅₀值为 108.5 μM。