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Cycling versus Continuous High Pressure treatments at moderate temperatures: Effect on bacterial spores?
Innovative Food Science & Emerging Technologies ( IF 6.6 ) Pub Date : 2021-09-09 , DOI: 10.1016/j.ifset.2021.102828
Fatima Fekraoui 1 , Éric Ferret 1 , Nathalie Paniel 2 , Olivia Auvy 1 , Clara Chamontin 2 , Stéphane André 2 , Hélène Simonin 1 , Jean-Marie Perrier-Cornet 1
Affiliation  

The advantage of using high pressure (HP) cycling treatment compared with continuous HP treatment was investigated for the inactivation of bacterial spores. The effects of parameters such as pulse number, pressure level, treatment temperature, compression and decompression rates, and time between pulses were evaluated. For this purpose, Bacillus subtilis and B. cereus spores (108 and 106 CFU/mL respectively) were suspended in 2-(N-morpholino) ethanesulfonic acid (MES) buffer solution, tryptone salt (TS) buffer solution, or infant milk and treated by HP cycling at 300–400 MPa, at 38–60 °C, for 1–5 pulses. Pressure cycling reduced the number of viable spores by 1.8 and 5.9 log respectively for B. subtilis and B. cereus species. Continuous HP treatments were performed at the same pressure and temperature for similar treatment durations. Our results showed that the spore inactivation ratio was correlated with the cumulative exposure time to pressure rather than to effects of the cycling process. Greater spore inactivation caused by HP cycling was observed only when faster compression and decompression rates were applied, probably due to adiabatic heating. A three-step kinetic model was developed, which seemed to support our hypothesis regarding the mechanisms of inactivation by pressure cycling and continuous HP treatments.

Industrial relevance

The resistance of bacterial spores to HP limits the industrial applications to refrigerated food products. In this study, we investigated the use of pressure cycling as a means to improve spore baroinactivation at moderate temperatures (T < 60 °C). We showed that cycling pressure does not significantly increase bacterial spore inactivation in comparable treatment duration, but certainly increases material fatigue in HP vessels. Thus, under moderate temperature, cycling pressure treatment is not industrially relevant.



中文翻译:

在中等温度下循环与连续高压处理:对细菌孢子的影响?

研究了使用高压 (HP) 循环处理与连续 HP 处理相比的优势,以灭活细菌孢子。评估了诸如脉冲数、压力水平、治疗温度、压缩和减压速率以及脉冲之间的时间等参数的影响。为此,将枯草芽孢杆菌蜡状芽孢杆菌孢子(分别为 10 8和 10 6  CFU/mL)悬浮在 2-(N-吗啉代)乙磺酸(MES)缓冲溶液、胰蛋白胨盐(TS)缓冲溶液或婴儿牛奶并在 300–400 MPa、38–60 °C、1–5 个脉冲的条件下进行 HP 循环处理。压力循环使枯草芽孢杆菌枯草芽孢杆菌的活孢子数量分别减少了 1.8 和 5.9 logB.蜡状种。连续高压处理在相同的压力和温度下进行相似的处理持续时间。我们的结果表明,孢子灭活率与累积暴露于压力的时间相关,而不是与循环过程的影响相关。仅当应用更快的压缩和减压速率时才观察到由 HP 循环引起的更大的孢子灭活,这可能是由于绝热加热。开发了一个三步动力学模型,这似乎支持我们关于压力循环和连续 HP 治疗灭活机制的假设。

工业相关性

细菌孢子对 HP 的抵抗力限制了冷藏食品的工业应用。在这项研究中,我们研究了使用压力循环作为在中等温度 ( T  < 60 °C)下改善孢子 baroinactivation 的一种手段。我们表明,在可比较的治疗时间内,循环压力不会显着增加细菌孢子的灭活,但肯定会增加 HP 容器中的材料疲劳。因此,在中等温度下,循环压力处理与工业无关。

更新日期:2021-10-12
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