The aim of this study was to investigate the respective contribution of maternally-inherited mRNAs and proteins to egg molecular cargo and to its developmental competence in fish using pikeperch as a model. Our study provides novel insights into the understanding of type-specific roles of maternally-inherited molecules in fish. Here we show, for the first time, that transcripts and proteins have distinct, yet complementary, functions in the egg of teleost fish. Maternally-inherited mRNAs would shape embryo neurodevelopment, while maternally-inherited proteins would rather be responsible for protecting the embryo against pathogens. Additionally, we observed that processes directly preceding ovulation may considerably affect the reproductive success by modifying expression level of genes crucial for proper embryonic development, being novel fish egg quality markers (e.g., smarca4 or h3f3a). These results are of major importance for understanding the influence of external factors on reproductive fitness in both captive and wild-type fish species.

本研究的目的是研究母系遗传的 mRNA 和蛋白质对卵子分子货物及其在鱼类发育能力中的各自贡献，以梭鲈为模型。我们的研究为理解鱼类中母系遗传分子的类型特异性作用提供了新的见解。在这里，我们首次展示了转录物和蛋白质在硬骨鱼卵中具有不同但互补的功能。母系遗传的 mRNA 会影响胚胎的神经发育，而母系遗传的蛋白质则更愿意负责保护胚胎免受病原体的侵害。此外，我们观察到直接在排卵之前的过程可能会通过改变对正常发育至关重要的基因的表达水平来显着影响生殖成功。胚胎发育，是新的鱼卵质量标记（例如smarca4或h3f3a ）。这些结果对于了解外部因素对圈养和野生鱼类繁殖适应性的影响具有重要意义。