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Characters of a nitrobacter enrichment culture from a freshwater aquaculture pond
Biotechnology & Biotechnological Equipment ( IF 1.4 ) Pub Date : 2021-09-09 , DOI: 10.1080/13102818.2021.1974944
Mengying He 1 , Ying Xiong 1 , Kai Cheng 1
Affiliation  

Abstract

To isolate nitrite-oxidizing bacteria to control the nitrite concentration in freshwater aquaculture systems, a Nitrobacter enrichment culture was obtained from a freshwater shrimp pond using a continuous enrichment and gradient dilution method. There was only one species of Nitrobacter in the enrichment culture, with an abundance of more than 99%. The Nitrobacter strain was identified as Nitrobacter vulgaris XY-12 on the basis of its 16S rRNA gene and NxrA gene sequences. The effects of pH, temperature, salinity and nitrate on the nitrification activity of the enrichment culture were examined, and its ability to remove nitrite in aquaculture water was also tested. The results showed that the optimum pH for the nitrite-oxidation activity of the enrichment culture was 7.5, the optimum temperature range was 27 °C–30 °C, the optimum salinity was 0.38%, the half maximal inhibitory concentration (IC50) of NO3–N was 400 mg L−1, and the optimum concentration of NO2–N was 200 mg L−1. In optimum conditions, the maximum NO2–N removal rate afforded by the enrichment was 1.62 mg L−1 h−1, and the minimum doubling time was 12 ± 2 h. Moreover, XY-12 could multiply in field aquaculture water, and the maximum NO2–N removal rate was 0.15 mg L−1 h−1. These results indicated that the enrichment had good application prospects in aquaculture waters.



中文翻译:

淡水养殖池塘硝化细菌富集培养特性

摘要

为了分离亚硝酸盐氧化细菌以控制淡水养殖系统中的亚硝酸盐浓度,使用连续富集和梯度稀释方法从淡水虾池中获得了硝化细菌富集培养物。富集培养中只有一种硝化杆菌,丰度超过99%。根据其16S rRNA基因和NxrA,硝化杆菌菌株鉴定为普通硝化杆菌XY-12基因序列。考察了pH、温度、盐度和硝酸盐对富集养殖硝化活性的影响,并测试了其去除水产养殖水中亚硝酸盐的能力。结果表明,富集培养物亚硝酸盐氧化活性的最适pH为7.5,最适温度范围为27℃~30℃,最适盐度为0.38%,半数抑制浓度(IC 50)为NO 3 - -N 为400 mg L -1,NO 2 - -N的最佳浓度为200 mg L -1。在最佳条件下,富集提供的最大NO 2 - -N去除率为1.62 mgL -1 h -1,最小倍增时间为12±2 h。此外,XY-12可以在野外养殖水中繁殖,最大NO 2 - -N去除率为0.15 mg L -1 h -1。这些结果表明富集在水产养殖水域具有良好的应用前景。

更新日期:2021-09-09
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