Abstract

Bovine viral diarrhea virus (BVDV) is a ubiquitous immunosuppressive etiological agent which is economically important for a wide host range in the livestock industry. Lactobacillus spp. has widely been using in the field of management and treatment of gastro-enteric disease for both humans and animals. The ability of Lacticaseibacillus casei MCJ protein-based metabolized to suppress BVDV infection in Madin–Darby Bovine Kidney cell line was demonstrated in this study. The protein-based metabolites were extracted from the cultured L. casei to obtain the safest and beneficial form of the probiotic bacteria. It is revealed that LPM have no cytotoxic effect and the cell viability remain more than 80% even after the cells are treated with 3000 µg/mL of LPM. The results of the plaque formation assay showed that LPM can reduce the viral infection rate. To know the mechanism of LPM for anti-BVDV activity, MDBK cells were exposed to LPM before, after and co-incubation of virus infection. The co-treatment of LPM with BVDV revealed the best results. The results suggest that the LPM has a potential anti-BVDV activity which could be a prospective candidate for the prevention and control of BVDV infection in an animal.

摘要

牛病毒性腹泻病毒 (BVDV) 是一种普遍存在的免疫抑制病原体，对于畜牧业中广泛的宿主范围具有重要的经济意义。乳酸菌属 已广泛应用于人类和动物胃肠疾病的管理和治疗领域。本研究证明了基于干酪乳杆菌MCJ 蛋白的代谢抑制 Madin-Darby 牛肾细胞系中 BVDV 感染的能力。从培养的干酪乳杆菌中提取基于蛋白质的代谢物以获得最安全和有益的益生菌形式。结果显示，LPM 没有细胞毒性作用，即使在用 3000 µg/mL LPM 处理细胞后，细胞活力仍保持在 80% 以上。空斑形成试验的结果表明 LPM 可以降低病毒感染率。为了解 LPM 抗 BVDV 活性的机制，将 MDBK 细胞在病毒感染之前、之后和共同孵育时暴露于 LPM。LPM 与 BVDV 的共同处理显示出最好的结果。结果表明，LPM 具有潜在的抗 BVDV 活性，可能成为预防和控制动物 BVDV 感染的潜在候选物。