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Research on the inhibition for aseptic loosening of artificial joints by Sr-doped calcium polyphosphate (SCPP) in vivo
Biomedical Materials ( IF 4 ) Pub Date : 2021-10-04 , DOI: 10.1088/1748-605x/ac2492
Xu Peng 1, 2 , Yanjiang Li 1 , Can Cheng 1 , Wang Ning 3 , Xixun Yu 1
Affiliation  

Aseptic loosening of artificial joints is the most common complication after artificial joint replacement. Finding the solution to tackle aseptic loosening of artificial joints is a focus in bone and joint surgery research field. In vitro studies of Sr-doped calcium polyphosphate (SCPP) have found by our team that it could promote osteoblast proliferation and inhibit osteoclast activity, and it has a potential inhibitory effect on aseptic loosening by suppressing the expression of receptor activator of nuclear factor-κ B ligand and improving the expression of OPG. The present study aims to confirm the conclusion in vitro by the mean of animal experiment. The Ti rod prosthesis coated with SCPP, calcium polyphosphate (CPP), and Ultra-high molecular weight polyethylene (UHMWPE were implanted in the femur (the internal surface of bone tunnel was also coated with SCPP, CPP and UHMWPE respectively). Radiography (x-rays, micro-CT), histochemistry (Hematoxylin-eosin staining (HE), methylene blue-acid fuchsin, Von Kossa histological staining), molecular biology (alkaline phosphatase and TRAP5b factors, Mir21-5p and Mir 26a-5p) were performed to analyzed the effects of SCPP within 20 weeks. The Radiography results showed that osteolysis with various severity occurred in all groups, and SCPP group had the mildest osteolysis. Histochemistry results showed that arthritis was milder in SCPP and CPP groups, while the bone formation in SCPP group was most significant. Its bone reconstruction effect was the best as well. The Molecular biology results showed that the bone reconstruction was out-sync in each group. Compared with other groups, the bone resorption occurred at the latest and the bone resorption time was the shortest in experimental animals of SCPP group. All results indicated that SCPP could promote osteoblast activity and bone reconstruction, improve the integration of bone interface between prosthesis and base bone, reduce osteoclast activity and shorten the osteoclast action time at the implantation site in vivo. Thus, it could postpone or alleviate the occurrence and development of aseptic loosening in vivo. Therefore, SCPP could be a promising material for the construction of artificial joints with the ability to resist aseptic loosening.



中文翻译:

掺锶聚磷酸钙(SCPP)体内抑制人工关节无菌性松动的研究

人工关节无菌性松动是人工关节置换术后最常见的并发症。寻找解决人工关节无菌性松动的方法是骨关节外科研究领域的热点。我们团队体外研究发现掺锶聚磷酸钙(SCPP)可以促进成骨细胞增殖,抑制破骨细胞活性,并通过抑制核因子-κ受体激活剂的表达,对无菌性松动具有潜在的抑制作用。 B配体并提高OPG的表达。本研究旨在通过动物实验的方式在体外证实该结论。将涂有 SCPP、聚磷酸钙(CPP)和超高分子量聚乙烯(UHMWPE)的钛棒假体植入股骨内(骨隧道内表面也分别涂有 SCPP、CPP 和 UHMWPE)。进行了射线、显微 CT)、组织化学(苏木精-伊红染色 (HE)、亚甲蓝酸品红、Von Kossa 组织学染色)、分子生物学(碱性磷酸酶和 TRAP5b 因子、Mir21-5p 和 Mir 26a-5p)分析20周内SCPP的效果,X线检查结果显示,各组均出现不同程度的骨质溶解,其中SCPP组骨质溶解最轻。组织化学结果显示,SCPP组和CPP组关节炎较轻,而骨形成则较轻。 SCPP组最为显着,骨重建效果最好,分子生物学结果显示各组骨重建不同步,与其他组相比,骨吸收发生最晚,骨吸收时间SCPP组实验动物中最矮。所有结果表明,SCPP在体内能够促进成骨细胞活性和骨重建,改善假体与基骨之间的骨界面整合,降低破骨细胞活性,缩短破骨细胞在体内植入部位的作用时间。从而延缓或减轻体内无菌性松动的发生和发展。因此,SCPP可能是一种有前途的用于构建具有抗无菌性松动能力的人工关节的材料。

更新日期:2021-10-04
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