Glucocorticoids-induced osteonecrosis of the femoral head (GIONFH) is a common refractory disease. In the present study, we aimed to synthesize the nano-hydroxyapatite-copper-lithium (Cu-Li-nHA) composite porous scaffold to promote osteogenesis and angiogenesis functions to repair GIONFH by regulating the Wnt/β-catenin and HIF-1α/VEGF pathways. The physicochemical property of the scaffold was characterized and their osteogenic and angiogenic effects were tested through a serial of experiments in vitro and in vivo. Results showed that 0.25% Cu-Li-nHA scaffolds possessed the highest mechanical and biocompatibility in vitro. Then the 0.25% Cu-Li-nHA scaffolds significantly enhanced the new bone formation on defects in GIONFH rabbits in vivo. Moreover, the scaffold could increase the expression of osteogenic and angiogenic factors along with the activation of factors in Wnt/β-catenin and HIF-1α/VEGF pathways in vitro and in vivo. In conclusion, the 0.25% Cu-Li-nHA scaffold could improve the osteogenesis and angiogenesis by upregulating the Wnt/β-catenin and HIF-1α/VEGF pathways which benefited to repair the GIONFH in rabbit models.

糖皮质激素诱导的股骨头坏死（GIONFH）是一种常见的难治性疾病。本研究旨在合成纳米羟基磷灰石-铜-锂（Cu-Li-nHA）复合多孔支架，通过调节Wnt/ β -catenin和HIF-1α /促进成骨和血管生成功能修复GIONFH。血管内皮生长因子通路。通过一系列体外和体内实验，对支架的理化性质进行了表征，并对其成骨和血管生成作用进行了测试。结果表明，0.25% Cu-Li-nHA 支架具有最高的体外机械和生物相容性. 然后，0.25% Cu-Li-nHA 支架显着增强了 GIONFH 兔体内缺陷处的新骨形成。此外，支架可以增加成骨和血管生成因子的表达，同时在体外和体内激活 Wnt/ β -catenin 和 HIF-1 α /VEGF 通路中的因子。总之，0.25% Cu-Li-nHA 支架可通过上调 Wnt/ β -catenin 和 HIF-1 α /VEGF 通路改善成骨和血管生成，有利于修复兔模型中的 GIONFH。