Euphorbia is the largest genus in family Euphorbiaceae with a great biological and genetic diversity. The anti-trypanosomal activity of the crude extract of 15 Euphorbia species against Trypanosoma brucei brucei was carried out. Furthermore, DNA fingerprinting of the tested species using ISSR and SCoT markers was also investigated. The anti-trypanosomal activity of the 15 Euphorbia species revealed the highest activity of E. officinarum L. and E. milli Des Moul. against Trypanosoma brucei brucei with IC50 values < 10 μg/mL after 48- and 72-h incubation. Moreover, the assessment of the genetic diversity among the 15 tested species showed similar correlation coefficients of 0.76–0.98 which classified Euphorbia species into two main groups, one contained two species and the other contained 13 species. Some Euphorbia species exhibited significant growth inhibitory activity toward Trypanosoma brucei strain TC221. Results also indicated the suitability of both markers for genetic fingerprinting of the tested Euphorbia species. To our knowledge, this is the first detailed comparison of the performance of two targeted DNA molecular markers (SCoT and ISSR) on the tested 15 Euphorbia species. The results guide future efficient use of these molecular markers in the genetic analysis of Euphorbia.

中文翻译：

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使用 ISSR 和 SCoT 标记物对 15 种大戟属植物的抗锥虫活性和 DNA 指纹图谱

大戟属是大戟科中最大的属，具有丰富的生物和遗传多样性。进行了15种大戟属植物粗提物对布氏锥虫的抗锥虫活性。此外，还研究了使用 ISSR 和 SCoT 标记对测试物种的 DNA 指纹图谱。15 种大戟属植物的抗锥虫活性显示 E. officinarum L. 和 E. mill Des Moul 的活性最高。培养 48 小时和 72 小时后，对布氏锥虫的 IC50 值 < 10 μg/mL。此外，对 15 个测试物种的遗传多样性的评估显示出相似的相关系数为 0.76-0.98，将大戟属物种分为两大类，一个包含 2 个物种，另一个包含 13 个物种。一些大戟属植物对布氏锥虫菌株 TC221 表现出显着的生长抑制活性。结果还表明两种标记都适用于测试的大戟属植物的遗传指纹图谱。据我们所知，这是对两种靶向 DNA 分子标记（SCoT 和 ISSR）在测试的 15 种大戟属植物上的性能的首次详细比较。结果指导未来在大戟属植物的遗传分析中有效使用这些分子标记。