Bacillus cereus is a notorious foodborne pathogen, which can grow under anoxic conditions. Anoxic growth is supported by endogenous redox metabolism, for which the thiol redox proteome serves as an interface. Here, we studied the cysteine (Cys) proteome dynamics of B. cereus ATCC 14579 cells grown under fermentative anoxic conditions. We used a quantitative thiol trapping method combined with proteomics profiling. In total, we identified 153 reactive Cys residues in 117 proteins participating in various cellular processes and metabolic pathways, including translation, carbohydrate metabolism, and stress response. Of these reactive Cys, 72 were detected as reduced Cys. The B. cereus Cys proteome evolved during growth both in terms of the number of reduced Cys and the Cys-containing proteins identified, reflecting its growth-phase-dependence. Interestingly, the reduced status of the B. cereus thiol proteome increased during growth, concomitantly to the decrease of extracellular oxidoreduction potential. Taken together, our data show that the B. cereus Cys proteome during unstressed fermentative anaerobic growth is a dynamic entity and provide an important foundation for future redox proteomic studies in B. cereus and other organisms.

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发酵厌氧生长过程中蜡样芽孢杆菌硫醇蛋白质组的氧化还原蛋白质组学研究

蜡状芽孢杆菌是一种臭名昭著的食源性病原体，它可以在缺氧条件下生长。内源性氧化还原代谢支持缺氧生长，其中硫醇氧化还原蛋白质组充当界面。在这里，我们研究了在发酵缺氧条件下生长的蜡状芽孢杆菌 ATCC 14579 细胞的半胱氨酸 (Cys) 蛋白质组动力学。我们使用了定量硫醇捕获方法与蛋白质组学分析相结合。总的来说，我们在参与各种细胞过程和代谢途径的 117 种蛋白质中鉴定了 153 个反应性 Cys 残基，包括翻译、碳水化合物代谢和应激反应。在这些反应性 Cys 中，72 个被检测为还原的 Cys。蜡样芽孢杆菌 Cys 蛋白质组在生长过程中在减少的 Cys 数量和鉴定的含有 Cys 的蛋白质方面进化，反映了其生长阶段依赖性。有趣的是，蜡状芽孢杆菌硫醇蛋白质组的降低状态在生长过程中增加，伴随着细胞外氧化还原电位的降低。总之，我们的数据表明，无压力发酵厌氧生长期间的蜡状芽孢杆菌 Cys 蛋白质组是一个动态实体，为未来蜡状芽孢杆菌和其他生物的氧化还原蛋白质组学研究提供了重要基础。