A highly sensitive electrochemical biosensors has been developed for the detection of multiplex micro ribonucleic acids (miRNAs) by modifying an electrode with reduced graphene oxide/poly(2-aminobenzylamine)/gold nanoparticles and adopting porous, hollow silver-gold nanoparticles as tagged labeling with metal ions. In addition, an anti-deoxyribonucleic acid (DNA)-RNA hybrid [S9.6] antibody was used to detect different hybridized capture DNAs and miRNAs that can detect multiple miRNAs simultaneously. The developed electrochemical platform exhibits high selectivity, stability, and sensitivity with a wide linear range from 1 fM to 10 nM and a low detection limit of 0.98 fM, 3.58 fM, and 0.25 fM for miRNA-155, miRNA-21, and miRNA-16, respectively. In addition, the proposed electrochemical biosensor capable for the simultaneous detection of miRNA-155, miRNA-16, and miRNA-21, which are breast cancer biomarkers, in normal human serum, can be adopted and potentially used for breast cancer screening.

Graphical abstract

中文翻译：

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用于同时多重检测用于乳腺癌筛查的 microRNA 的电化学生物传感器的开发

通过用还原的氧化石墨烯/聚（2-氨基苄胺）/金纳米粒子修饰电极并采用多孔中空银-金纳米粒子作为标记标记，开发了一种用于检测多重微核糖核酸（miRNA）的高灵敏度电化学生物传感器。金属离子。此外，抗脱氧核糖核酸 (DNA)-RNA 杂交体 [S9.6] 抗体用于检测不同的杂交捕获 DNA 和 miRNA，可同时检测多个 miRNA。所开发的电化学平台具有高选择性、稳定性和灵敏度，线性范围从 1 fM 到 10 nM，对 miRNA-155、miRNA-21 和 miRNA-155、miRNA-21 和 miRNA-的检测限低至 0.98 fM、3.58 fM 和 0.25 fM。 16，分别。此外，所提出的电化学生物传感器能够同时检测 miRNA-155，

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