Exfoliated magadiite nanosheets embedded with Fe₃O₄ were constructed. Advantage was taken of the strong coordination between the silanol groups in magadiite nanosheets and the Gd³⁺ ion to prepare the final adsorbent, Gd³⁺-immobilized magnetic magadiite nanosheets. The adsorbent with two-dimensional (2D) morphology offered high surface area and abundant Gd³⁺ contents for phosphopeptides enrichment, on which Fe₃O₄ with positive electricity incorporated the magnetic properties. Combining with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF-MS), the method showed low detection limit (0.05 fmol). The feasibility of using the 2D nanocomposite for phosphopeptides enrichment was demonstrated using mixtures of β-casein and bovine serum albumin (1:5000). The standard deviation of captured phosphopeptides in three repeated experiments were in the range 0.15–0.42 (< 0.5% RSD). Further evaluation revealed that the nanocomposite was capable of enriching phosphopeptides from non-fat milk, human saliva, and serum.

Graphical abstract

中文翻译：

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Gd3+-固定二维磁性magadiite纳米片的设计用于磷酸肽的高选择性富集

_{构建了嵌入Fe 3} O ₄的剥离magadiite纳米片。利用magadiite纳米片中的硅烷醇基团与Gd ³⁺离子之间的强配位优势，制备了最终的吸附剂Gd ³⁺固定磁性magadiite纳米片。具有二维 (2D) 形态的吸附剂提供了高表面积和丰富的 Gd ³⁺含量，用于富集磷酸肽，Fe ₃ O ₄与正电结合的磁性。结合基质辅助激光解吸电离飞行时间质谱（MALDI TOF-MS），该方法检测限低（0.05 fmol）。使用β-酪蛋白和牛血清白蛋白 (1:5000)的混合物证明了使用 2D 纳米复合材料富集磷酸肽的可行性。在三个重复实验中捕获的磷酸肽的标准偏差在 0.15–0.42 范围内（< 0.5% RSD）。进一步的评估表明，纳米复合材料能够从脱脂牛奶、人唾液和血清中富集磷酸肽。

图形概要