Toona ciliata has considerable economic value, providing high-quality materials and medicines. However, the growth and sustainable development of T. ciliata is limited due to environmental changes, poor natural regeneration ability, and excessive logging. Therefore, this study used sterile seedling leaves from a good provenance of T. ciliata as explants to establish a highly efficient regeneration and genetic transformation system. The effects of different hormones and concentrations on leaf callus induction were investigated. A genetic transformation system using kanamycin (Kan) as the selective agent was established based on the regeneration system. The results showed that Murashige and Skoog (MS) medium supplemented with 3 mg/L N-(Phenylmethyl)-9H-purin-6-amine (6-BA), 1 mg/L kinetin (KT) and 0.05 mg/L 1-naphthlcetic acid (NAA) (T1 medium) were suitable for callus formation and adventitious bud production from whole leaves at 45 days. In addition, pCAMBIA2301-EYFP was introduced into competent cells of Agrobacterium tumefaciens strain GV3101 and used for genetic transformation. Healthy leaves pre-cultured on T1 medium for three days were directly infected with A. tumefaciens infection solution (OD₆₀₀ = 0.6) for 20 min through the addition of 50 mmol/L of Acetosyringone (AS). Thereafter, the infected leaves were co-cultivated for one day on T1 medium supplemented with 150 mmol/L AS, and the infected leaves were transferred to fresh T1 medium containing 10 mg/L Kan and 100 mg/L Cefotaxime (Cef). Twenty-four positive transformed plants were selected through histological staining and PCR, laser confocal microscopy of EYFP from 259 putative transformants was conducted, and the transformed plants were obtained after approximately three months.

香椿具有可观的经济价值，可提供优质原料和药物。然而，由于环境变化、自然再生能力差、过度采伐等原因，纤毛虫的生长和可持续发展受到限制。因此，本研究使用了来自T. ciliata良好种源的不育苗叶作为外植体，建立高效的再生和遗传转化系统。研究了不同激素和浓度对叶愈伤组织诱导的影响。在再生系统的基础上建立了以卡那霉素(Kan)为选择剂的遗传转化系统。结果表明，Murashige 和 Skoog (MS) 培养基添加了 3 mg/L N-(Phenylmethyl)-9H-purin-6-amine (6-BA)、1 mg/L 激动素 (KT) 和 0.05 mg/L 1萘酸 (NAA)（T1 培养基）适合在 45 天时从全叶形成愈伤组织和产生不定芽。此外，将 pCAMBIA2301-EYFP 导入根癌农杆菌感受态细胞菌株 GV3101 并用于遗传转化。在 T1 培养基上预培养三天的健康叶片通过添加 50 mmol/L 乙酰丁香酮 (AS)直接感染根癌农杆菌感染溶液 (OD ₆₀₀ = 0.6) 20 分钟。此后，将感染的叶子在补充有 150 mmol/L AS 的 T1 培养基上共培养一天，并将感染的叶子转移到含有 10 mg/L Kan 和 100 mg/L 头孢噻肟 (Cef) 的新鲜 T1 培养基中。通过组织学染色和PCR筛选出24株阳性转化植株，对259株推定的转化株进行EYFP激光共聚焦显微镜观察，约3个月后获得转化植株。