Cocaine (Coc) is one of the illegal drugs and is harmful to digestive, immune, cardiovascular and urogenital systems. To achieve drug abuse control and legal action, it is essential to develop an effective method for cocaine analysis. In this work, an aptasensor has been developed using atom transfer radical polymerization (ATRP) based on host−guest chemistry for electrochemical analysis of cocaine. The NH₂−DNA (Apt1) was immobilized on the indium tin oxide (ITO) electrode via addition reaction, and Fc−DNA (Apt2) was introduced to ITO relying on the specific recognition of cocaine. The Apt2 can initiate host−guest chemistry between Apt2 and ATRP initiators (β−CD−Br₁₅), then the β−CD−Br₁₅ further triggers ATRP. Moreover, ATRP avoids the sluggish kinetics and poor coupling capability sustained. The result shows a sensitive and selective analysis of cocaine within a linear range from 0.1 ng/mL to 10 μg/mL (R² = 0.9985), with the detection limit down to 0.0335 ng/mL. Thus, this strategy provides a universal method for the analysis of illegal drugs.

可卡因 (Coc) 是非法药物之一，对消化、免疫、心血管和泌尿生殖系统有害。为了实现药物滥用控制和法律行动，开发一种有效的可卡因分析方法至关重要。在这项工作中，使用基于主客体化学的原子转移自由基聚合（ATRP）开发了一种适体传感器，用于可卡因的电化学分析。NH ₂ -DNA (Apt1) 通过加成反应固定在氧化铟锡 (ITO) 电极上，依靠可卡因的特异性识别将 Fc-DNA (Apt2) 引入 ITO。Apt2 可以启动 Apt2 和 ATRP 引发剂 (β-CD-Br ₁₅ )之间的宿主-客体化学反应，然后是 β-CD-Br ₁₅进一步触发 ATRP。此外，ATRP 避免了缓慢的动力学和持续的耦合能力差。结果表明，可卡因在 0.1 ng/mL 至 10 μg/mL (R ²  = 0.9985)的线性范围内进行了灵敏且选择性的分析，检测限低至 0.0335 ng/mL。因此，该策略为分析非法药物提供了一种通用方法。