Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products. Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation. CME's composition and availability suggest a potential carbon source for cellulose production. CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus. CME can be used to produce BC. However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields. BC production increased from 0.55 ± 0.16 g L⁻¹ up to 13.13 ± 1.09 g L⁻¹ after CME dilution and addition of a nitrogen source. BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.

在全球范围内，只有 8% 的可可果实生物质被用作巧克力产品中的可可豆。可可粘液分泌物 (CME) 是一种营养丰富的液体，通常会在可可豆发酵过程中流失。CME 的组成和可用性表明纤维素生产的潜在碳源。使用 CME 和 Hestrin 和 Schramm 培养基作为生长培养基，用木糖醋杆菌生产细菌纤维素 (BC )。CME 可用于生产 BC。然而，CME 中的高糖含量、低 pH 值和有限的氮源会阻碍G. xylinus 的生长，从而影响纤维素产量。BC 产量从 0.55 ± 0.16 g L ^-1增加到 13.13 ± 1.09 g L ^-1在 CME 稀释和添加氮源后。BC 生产从 30 毫升扩大到 15 升，使用实验室规模的实验条件，产量和生产率没有显着变化，表明具有工业可能性的稳健工艺。