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Microemulsion Preconcentration of Steroid Hormones from Aqueous Solutions and Urine Samples
Journal of Analytical Chemistry ( IF 1.1 ) Pub Date : 2021-09-07 , DOI: 10.1134/s1061934821090057
L. A. Kartsova 1 , S. A. Solov’eva 1 , E. A. Bessonova 1
Affiliation  

Abstract

A possibility of using an oil-in-water microemulsion of the composition 3% of sodium dodecyl sulfate, 8% of n-butanol, 1% of ethyl acetate, and 88% of water (by weight) as an extractant for the extraction and preconcentration of steroid hormones cortisol, cortisone, corticosterone, 11-deoxycortisol, and 11‑deoxycorticosterone from aqueous solutions and urine samples followed by their reversed-phase HPLC analysis with spectrophotometric detection is shown. In optimizing the extraction conditions, the nature of the oil, the time and temperature of extraction, and the time of the separation of the microemulsion were varied. Magnesium chloride was used to destroy the microemulsion. The concentration factors for steroid hormones increase with an increase in the hydrophobicity of the analytes. The limits of detection for steroids were 0.5–3 ng/mL, and concentration factors were 8–20.



中文翻译:

水溶液和尿液样品中类固醇激素的微乳液预浓缩

摘要

使用由 3% 十二烷基硫酸钠、8% n组成的水包油微乳液的可能性-丁醇、1% 的乙酸乙酯和 88% 的水(按重量计)作为提取剂,用于从水溶液和尿样中提取和预浓缩类固醇激素皮质醇、可的松、皮质酮、11-脱氧皮质醇和 11-脱氧皮质酮通过他们的反相 HPLC 分析与分光光度检测显示。在优化提取条件时,油的性质、提取的时间和温度以及微乳的分离时间是不同的。氯化镁用于破坏微乳液。类固醇激素的浓度因子随着分析物疏水性的增加而增加。类固醇的检测限为 0.5-3 ng/mL,浓度因子为 8-20。

更新日期:2021-09-08
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