ABSTRACT

The aim of this survey was to fabrication of chitosan nanoparticle was loaded by Boswellia sacra essential oil (BS-CNPs) and investigation of its pharmacological activities.BS-CNPs was synthesized by ionic gelation method and characterized. BS-CNPs-mediated cell viability and cell death were studied by MTT, qPCR, AO/PI staining and flow cytometery. Anti-angiogenesis properties were evaluated by CAM and scratch assay. Anti-oxidant properties were assessed by ABTS, DPPH, FRAP and ROS assay. The BS-CNPs (80.13 nm, ζ-p 39.20 mv and %EE: 82.7%) exhibited selective cytotoxicity on HepG2 cells (IC₅₀:62.94 μg/ml). BS-CNPs induced the apoptosis by up-regulation of P53, Cas-3 and 9. Increasing the rate of free radical scavenging and intracellular ROS confirmed the anti and pro-oxidant potential of BS-CNPs outside and inside of cancer cells.Inhibition (92.3%) of cell migration, reduction of angiogenic factors showed anti-angiogenic effects of BS-CNPs.BS-CNPs due tounique anti-cancer properties are suitable for therapeutic targets in cancer studies.

摘要

本研究旨在以乳香精油为载体制备壳聚糖纳米颗粒（BS-CNPs）并考察其药理活性。采用离子凝胶法合成并表征了BS-CNPs 。通过 MTT、qPCR、AO/PI 染色和流式细胞仪研究 BS-CNPs 介导的细胞活力和细胞死亡。通过CAM和划痕试验评估抗血管生成特性。通过 ABTS、DPPH、FRAP 和 ROS 测定评估抗氧化特性。BS-CNP（80.13 nm，ζ-p 39.20 mv 和 %EE：82.7%）对 HepG2 细胞表现出选择性细胞毒性（IC ₅₀:62.94 微克/毫升)。BS-CNPs 通过上调 P53、Cas-3 和 9 诱导细胞凋亡。增加自由基清除率和细胞内 ROS 证实了 BS-CNPs 在癌细胞内外的抗氧化和促氧化潜力。 92.3%)的细胞迁移，血管生成因子的减少显示出BS-CNPs的抗血管生成作用。BS-CNPs由于独特的抗癌特性，适用于癌症研究中的治疗靶点。