Group B Streptococcus (GBS) is a leading cause of neonatal meningitis, pneumonia, and sepsis. The biggest contributing factor of neonatal infections is due to vertical transmission from maternal colonisation of GBS in the genitourinary tract. Multiple serotype colonisation is often not investigated in epidemiological studies, but it is an important consideration for serotype-based vaccine development and implementation to ensure less abundant serotypes are not under-represented. In this study, we show that RAPD PCR is a quick tool useful in screening the presence of genetically different strains using multiple colony picks from a single patient swab. We observed a maximum of five different GBS strains colonising a single patient at a specific time.

B 组链球菌(GBS) 是导致新生儿脑膜炎、肺炎和败血症的主要原因。新生儿感染的最大影响因素是由于母体在泌尿生殖道中定植 GBS 的垂直传播。多血清型定植通常不会在流行病学研究中进行调查，但它是基于血清型的疫苗开发和实施的重要考虑因素，以确保不太丰富的血清型不会被低估。在这项研究中，我们表明 RAPD PCR 是一种快速工具，可用于使用来自单个患者拭子的多个菌落挑选来筛选遗传不同菌株的存在。我们观察到最多五种不同的 GBS 菌株在特定时间定殖单个患者。