How the three-dimensional (3D) chiral environment affects the biocatalysis remains an important issue, thereby inspiring the development of a microenvironment that highly mimics the natural features of enzyme to guarantee enhanced biocatalysis. In this study, two gelators bearing d/l-phenylalanine as chiral centers are designed to construct the 3D chiral catalytic microenvironment for enhancing the biocatalysis of lipase. Such a microenvironment is programmed through chiral transmission of chirality from molecular chirality to achiral polymers. It shows that the chirality of the microenvironment evidently influences the catalytic efficiency of immobilized lipase inside the system, and the 3D microenvironment constructed by right-handed helical nanostructures can enhance the catalytic activity of lipase inside as high as 10-fold for catalyzing 4-nitrophenyl palmitate (NPP) to 4-nitrophenol (NP) and 1.4-fold for catalyzing lipids to triglycerides (TGs) in 3T3-L1 cells than that of the achiral microenvironment. Moreover, the 3D chiral microenvironment has the merits of good catalytic efficiency, high storage stability, and efficient recyclability. This strategy of designing a 3D chiral microenvironment suitable for biocatalysis will overcome the present limitations of enzymatic immobilization in traditional materials and enhance the understanding of biocatalysis.

中文翻译：

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增强生物催化的三维手性超分子微环境策略

三维 (3D) 手性环境如何影响生物催化仍然是一个重要问题，从而激发了高度模仿酶的自然特征的微环境的发展，以保证增强的生物催化。在这项研究中，两个凝胶剂轴承d/l-苯丙氨酸作为手性中心旨在构建3D手性催化微环境以增强脂肪酶的生物催化作用。这种微环境是通过手性从分子手性到非手性聚合物的手性传递来编程的。表明微环境的手性显着影响系统内固定化脂肪酶的催化效率，右手螺旋纳米结构构建的3D微环境可将内部脂肪酶催化4-硝基苯的催化活性提高10倍棕榈酸酯 (NPP) 转化为 4-硝基苯酚 (NP) 并且在 3T3-L1 细胞中催化脂质转化为甘油三酯 (TGs) 是非手性微环境的 1.4 倍。此外，3D手性微环境具有催化效率好、储存稳定性高、和高效的可回收性。这种设计适合生物催化的 3D 手性微环境的策略将克服目前传统材料中酶固定化的局限性，并增强对生物催化的理解。