We studied the water quality of the riparian firefly sanctuary of Sungai Rembau, or Rembau River, in Negeri Sembilan, Malaysia, from January 2018 to November 2018 to determine the possible influence of the physico-chemical characteristics of the water on the firefly populations living within the sanctuary. We set up a total of five water quality sampling stations and 10 firefly sampling stations along the river. Dissolved oxygen (DO), temperature, pH and electrical conductivity (EC) were measured in situ, while chemical oxygen demand (COD), total suspended solids (TSS), biochemical oxygen demand (BOD) and ammonia–nitrogen (NH₃-N) were analysed in the laboratory. Firefly samples were collected using a sweep net at both day and night for 1 min. Sungai Rembau was categorized as Class II on the Malaysian water quality index (WQI), which indicates slight pollution. Except for EC and DO, the water quality parameter values were not significantly different (p > 0.05) between the sampling stations. A total of 529 firefly individuals consisting of Pteroptyx tener (n = 525, 99.24%), P. malaccae (n = 3, 0.57%) and P. asymmetria (n = 1, 0.19%) were collected. There was significant correlation between firefly abundance and BOD (r = − 0.198, p < 0.05), NH₃-N (r = − 0.150, p < 0.05) and pH (r = 0.408, p < 0.05). The results show that the firefly populations in Sungai Rembau are sensitive to organic compounds, which may be present in the form of pollutants from anthropogenic activities near their natural habitat.

我们研究了 2018 年 1 月至 2018 年 11 月马来西亚森美兰州双溪林堡河岸萤火虫保护区的水质，以确定水的物理化学特征对生活在其中的萤火虫种群的可能影响。圣所。沿河共设置5个水质采样站和10个萤火虫采样站。原位测量溶解氧 (DO)、温度、pH 值和电导率 (EC)，而化学需氧量 (COD)、总悬浮固体 (TSS)、生化需氧量 (BOD) 和氨氮 (NH ₃-N) 在实验室进行分析。萤火虫样本在白天和晚上使用扫网收集 1 分钟。双溪林堡在马来西亚水质​​指数 (WQI) 中被归类为 II 级，表明污染轻微。除EC和DO外， 各采样站的水质参数值均无显着差异（p >0.05）。共收集了 529 只萤火虫个体，包括Pteroptyx tener ( n  = 525, 99.24%)、  P. malaccae ( n  = 3, 0.57%) 和P. asymmetria (n = 1, 0.19%)。萤火虫丰度与 BOD 之间存在显着相关性 ( r  = − 0.198, p  < 0.05)、NH₃ -N ( r  = − 0.150, p  < 0.05) 和 pH ( r  = 0.408, p  < 0.05)。结果表明，双溪林堡的萤火虫种群对有机化合物很敏感，这些有机化合物可能以其自然栖息地附近的人为活动污染物的形式存在。